What question did this study set out to answer?

To improve gene editing efficiency in human iPSCs for organoid engineering using a nanopuncturing technique.

Nanopuncturing‐Enabled Efficient Gene Editing for Isogenic Human iPSC‐Derived Organoid Engineering

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Nanopuncturing enhances gene editing and knockout efficiency in iPSC-derived organoids, suggesting advances in disease modeling.

To improve gene editing efficiency in human iPSCs for organoid engineering using a nanopuncturing technique.
Utilized a nanoneedle-assisted nanopuncturing strategy for CRISPR/Cas9 plasmid delivery.
Evaluated transfection efficiency and gene knockout effects in hiPSCs.
Constructed isogenic organoid models by targeting specific genes.
Achieved 45 ± 4% transfection efficiency, significantly higher than traditional methods.
Constructed organoids with successful knockout of PTPRB and ZNF521 genes.
Preserved stemness and differentiation potential of engineered organoids.

Ghani et al. (2025) studied this question.

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View Full Paper

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Authors

Nanopuncturing enhances gene editing and knockout efficiency in iPSC-derived organoids, suggesting advances in disease modeling.

To improve gene editing efficiency in human iPSCs for organoid engineering using a nanopuncturing technique.
Utilized a nanoneedle-assisted nanopuncturing strategy for CRISPR/Cas9 plasmid delivery.
Evaluated transfection efficiency and gene knockout effects in hiPSCs.
Constructed isogenic organoid models by targeting specific genes.
Achieved 45 ± 4% transfection efficiency, significantly higher than traditional methods.
Constructed organoids with successful knockout of PTPRB and ZNF521 genes.
Preserved stemness and differentiation potential of engineered organoids.

Ghani et al. (2025) studied this question.

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