What is the clinical evidence from this study?

Study design: Other. Population: Cardiomyocyte cell cycle arrest. Intervention: Non-coding RNAs (STARD4-AS1, miR-372). Primary outcome: Cardiomyocyte cell cycle arrest and ncRNA expression.

October 9, 2025Circulation Research

Abstract Thu021: Regulatory Role of Non-Coding RNAs in Cardiomyocyte Cell Cycle Arrest and Postnatal Maturation

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Key result

STARD4-AS1 and miR-372 expression links to cardiomyocyte cell cycle arrest during differentiation.

Population

Human pluripotent stem cells (hPSCs) differentiated into cardiomyocytes

Comparison

Quantification of candidate ncRNA expression… vs Earlier time points and undifferentiated hPSCs

Design

Preclinical

Follow-up

30 days of differentiation

Authors

MRMalihe RezaeeJohns Hopkins University MSMahshad ShiriRoyan Institute FMFatemeh MovahediRoyan Institute

Discussion

Member takes

Implication

The long non-coding RNA STARD4-AS1 and microRNA miR-372 show dynamic expression during cardiomyocyte differentiation, suggesting a potential regulatory role in cell cycle arrest.

Key Points

Four lncRNAs and three miRNAs were identified as potential regulators of cardiomyocyte cell cycle arrest.
The study found significant downregulation of STARD4-AS1 at day 30 of differentiation, aligning with predictions from bioinformatics analysis.
Flow cytometry analysis showed increased G1-phase cardiomyocytes at day 30, indicating progression in postnatal maturation.
Further investigations are needed to thoroughly understand the functional roles of these non-coding RNAs in cardiac development.

Structured PICO

Population

Human pluripotent stem cells (hPSCs) differentiated into cardiomyocytes

Exposure

Quantification of candidate ncRNA expression (STARD4-AS1, H19, LINC1, LINC2, miR-302d, miR-371-5p, miR-372) during differentiation

Comparator

Earlier time points (days 10, 20) and undifferentiated hPSCs

Outcome

Expression levels of candidate ncRNAs and cell cycle phase distribution (G1, S, G2) at days 10, 20, and 30 of differentiationsurrogate

The long non-coding RNA STARD4-AS1 and microRNA miR-372 show dynamic expression during cardiomyocyte differentiation, suggesting a potential regulatory role in cell cycle arrest.

Limitations

Further studies are needed to determine its role in postnatal cardiac maturation.
Further studies are needed to determine its role in postnatal cardiac maturation

Cite This Study

Rezaee et al. (2025) studied Cardiomyocyte cell cycle arrest. Non-coding RNAs (STARD4-AS1, miR-372) was evaluated on Cardiomyocyte cell cycle arrest and ncRNA expression. STARD4-AS1 and miR-372 were identified as dynamically expressed non-coding RNAs that may regulate cardiomyocyte cell cycle arrest during differentiation.

synapsesocial.com/papers/68e77f09d1c187e1c108fc06 https://doi.org/10.1161/res.137.suppl_1.thu021

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Implication

The long non-coding RNA STARD4-AS1 and microRNA miR-372 show dynamic expression during cardiomyocyte differentiation, suggesting a potential regulatory role in cell cycle arrest.

Abstract Thu021: Regulatory Role of Non-Coding RNAs in Cardiomyocyte Cell Cycle Arrest and Postnatal Maturation

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Abstract Thu021: Regulatory Role of Non-Coding RNAs in Cardiomyocyte Cell Cycle Arrest and Postnatal Maturation

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider