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November 19, 2025eLifeOpen Access

Conduction pathway for potassium through the Escherichia coli pump KdpFABC

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Authors

AHAdel HusseinXZXihui ZhangBPBjørn P. Pedersen

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Overview

Research reveals potassium conduction through a hydrophobic tunnel in KdpFABC, implying its role in maintaining the electrochemical gradient.

Key Points

  • The aim is to investigate the conduction pathway for potassium ions through the KdpFABC pump in Escherichia coli.
  • Reconstitution of KdpFABC into lipid nanodiscs
  • Use of cryo-EM to image the pump structure under turnover conditions
  • ATPase and ion transport assays to evaluate mutations along the conduction pathway.
  • Identified a 2.1 Å structure of the E1~P·ADP conformation
  • Confirmed potassium ions pass through a hydrophobic tunnel
  • Supported existence of a low-affinity site in KdpB for ion release.

Cite This Study

Hussein et al. (2025) studied this question.

synapsesocial.com/papers/6924f095c0ce034ddc350cabhttps://doi.org/10.7554/elife.107397.4
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Conduction pathway for potassium through the E. coli pump KdpFABC2025 · 2 citations
  2. 2On the mechanism of K+ transport through the inter-subunit tunnel of KdpFABC2025 · 1 citations
  3. 3BPS2026 – Lipids play a functional role in the potassium transport by KdpFABC2026
  4. 4Numerical model for electrogenic transport by the ATP-dependent potassium pump KdpFABC2024 · 11 citations
  5. 5KdpD is a tandem serine histidine kinase that controls K+ pump KdpFABC transcriptionally and post-translationally2024 · 14 citations