HPLC, in particular, offers high resolution, accuracy, and reproducibility, making it indispensable for routine quality control and advanced research applications. Method development in HPLC involves systematic selection and optimization of mobile phase composition, column chemistry, detection wavelength, and operational parameters to achieve reliable separation and quantification. Analytical method validation, guided by ICH and USP standards, ensures that developed methods meet essential criteria, including accuracy, precision, linearity, specificity, robustness, and sensitivity. Keywords: Lasmiditan, Development, RP-HPLC, Accuracy, linearity, Limit of detection (LOD) and Limit of quantification (LOQ).
Darade et al. (Thu,) studied this question.