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March 8, 2026

CRISPR-Enhanced β-Carotene Biosynthesis: Droplet Microfluidics-Driven Pathway Optimization and High-Yield Terpenoid Strain Screening.

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Authors

SGShuilian GuoZLZhengjiao LiangSLShangfeng Liao

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Overview

Microfluidic screening enhanced β-carotene production in engineered yeast, suggesting new biosynthetic targets.

Key Points

  • To develop a microfluidic platform for optimizing β-carotene biosynthesis in yeast strains.
  • Created a microfluidic screening platform using inherent fluorescence of β-carotene.
  • Engineered yeast strain YsL4 with an enhanced mevalonate pathway was utilized for screening.
  • Conducted fluorescence-activated droplet sorting on a CRISPRa/i library to identify target genes.
  • Achieved 100% phenotypic specificity in coencapsulation assays between producer and nonproducer strains.
  • Identified 15 target genes, with 10 showing significant enhancements in β-carotene production.
  • Key mutant YL19 (ΔHMO1) produced 33.71 mg/L of β-carotene, a 149.15% increase.

Cite This Study

Guo et al. (2026) studied this question.

synapsesocial.com/papers/69acc5bd32b0ef16a4050694https://doi.org/10.1021/acs.jafc.5c10513
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