What question did this study set out to answer?

To evaluate the immunogenicity and protective efficacy of isolated foot-and-mouth disease virus capsid proteins in swine and guinea pigs.

December 1, 1975The Journal of ImmunologyOpen Access

Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus

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Why the study?

Does isolated capsid protein VP3 of foot-and-mouth disease virus elicit protective immune and antibody responses in swine and guinea pigs?

Population

Swine and guinea pigs

Comparison

Purified capsid proteins VP1, VP2, and VP3 of… vs VP1, VP2, mixtures of VP1/VP2/VP3, VP3 from…

Design

Preclinical

Follow-up

82 days

Authors

HBHoward L. BachrachAgricultural Research Service DMDouglas M. MooreJet Propulsion Laboratory PMP. D. McKercherThe Pirbright Institute

Discussion

Member takes

Implication

Informs VP3 antigen selection for FMD vaccines in swine; hypothesis-generating pending randomized livestock trials.

Key Points

To evaluate the immunogenicity and protective efficacy of isolated foot-and-mouth disease virus capsid proteins in swine and guinea pigs.
Inoculated swine and guinea pigs with purified capsid proteins VP1, VP2, or VP3 emulsified in incomplete Freund's adjuvant, testing single-protein, combined, and site-separated regimens.
Challenged vaccinated swine with live virus exposure on day 82 and evaluated antibody responses against intact, chemically inactivated, and trypsin-cleaved viral proteins.
Swine inoculated on days 0, 28, and 60 with 100-µg doses of VP3 developed viral precipitating and neutralizing antibodies and achieved protection against exposure to infected swine by day 82.
VP1 and VP2 failed to induce detectable antibodies in guinea pigs and diminished the anti-VP3 antibody response in swine when co-administered as a mixture at the same injection site.
VP3 derived from acetylethylenimine-treated virus showed reduced protective capacity, while cleaved fragments VP3a and VP3b did not induce detectable antibodies directly but primed animals for subsequent viral challenge.

Structured PICO

Does isolated capsid protein VP3 of foot-and-mouth disease virus elicit protective immune and antibody responses in swine and guinea pigs?

Population

Swine and guinea pigs

Intervention

Purified capsid proteins VP1, VP2, and VP3 of foot-and-mouth disease virus type A12 strain 119 emulsified with incomplete Freund's adjuvant (specifically VP3 at 100-mug doses on days 0, 28, and 60)

Comparator

VP1, VP2, mixtures of VP1/VP2/VP3, VP3 from acetylethylenimine-treated virus, trypsinized virus, and VP3a-VP3b mixture

Outcome

Protection against exposure to infected swine and production of viral precipitating and neutralizing antibodiessurrogate

Isolated VP3 capsid protein of foot-and-mouth disease virus can induce protective immunity and neutralizing antibodies in swine.

Cite This Study

Bachrach et al. (1975) studied this question.

synapsesocial.com/papers/69d6c2008dca315383ed8d22 https://doi.org/10.4049/jimmunol.115.6.1636

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Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider