Key result
Immunization with 40 or 45 residue synthetic peptides containing both 141-158 and 200-213 sequences of VP1 provided the most effective protection against foot-and-mouth disease virus in guinea-pigs.
Synthetic peptides containing specific VP1 sequences (141-158 and 200-213) with minimal additional amino acids provide the most effective protection against Foot-and-Mouth Disease in guinea-pigs.
Supports peptide optimization for FMDV; leaves open field efficacy in cattle and pigs.
Foot-and-mouth disease virus challenge experiments in guinea-pigs and immunoassays with a range of peptides equivalent to either or both of the sequences 141 to 158 and 200 to 213 of VP1 showed the most effective structure, in terms of protection, to be one in which both 'sites' were present with a minimum of additional amino acids. An 80 residue peptide comprising amino acids 134 to 213 was considerably less effective than 40 or 45 residue peptides. The major site for the induction of protection was deduced to be in the region 141 to 158. Thus, protection with the 40 or 45 residue peptide did not appear to be due to the presence of antibody directed solely to the 200 to 213 sequence. Finally, induction of antibody to the latter site appeared to be dependent on both the size of the peptide and the disposition of 'sites' within it.
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Doel et al. (1988) studied Foot-and-mouth disease. Synthetic peptides representing the C-terminal region of VP1 vs. Other peptide lengths (e.g., 80 residue peptide) was evaluated on Protection against foot-and-mouth disease virus challenge. Immunization with 40 or 45 residue synthetic peptides containing both 141-158 and 200-213 sequences of VP1 provided the most effective protection against foot-and-mouth disease virus in guinea-pigs.
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