What question did this study set out to answer?

To determine whether vascular endothelial cells actively facilitate the transport of insulin from blood vessels to surrounding tissues via a receptor-dependent mechanism.

March 29, 1985Science

Receptor-Mediated Transport of Insulin Across Endothelial Cells

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Authors

GKGeorge L. KingVascular Medicine SJSandra Marchese JohnsonUniversity of North Carolina at Chapel Hill

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Implication

In vitro study demonstrates receptor-mediated transport of intact insulin in bovine endothelial cells, suggesting active vascular regulation of hormone delivery.

Key Points

To determine whether vascular endothelial cells actively facilitate the transport of insulin from blood vessels to surrounding tissues via a receptor-dependent mechanism.
Cultured a horizontal monolayer of bovine aortic endothelial cells separating dual chambers to directly quantify transcellular movement.
Measured the transport of iodine-125-labeled insulin under variable temperatures and competitive conditions using unlabeled insulin and insulin-receptor antibodies.
Bovine aortic endothelial cells internalized and transported iodine-125-labeled insulin across the cell monolayer, with more than 80 percent of the transported insulin remaining intact.
Insulin transcellular transport was temperature sensitive and inhibited by unlabeled insulin and insulin-receptor antibodies in direct proportion to their ability to block receptor binding.

Cite This Study

King et al. (1985) studied this question.

synapsesocial.com/papers/69d7d8ec05ee2ba81dbee5ab https://doi.org/10.1126/science.3883490

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Implication

In vitro study demonstrates receptor-mediated transport of intact insulin in bovine endothelial cells, suggesting active vascular regulation of hormone delivery.

Receptor-Mediated Transport of Insulin Across Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Receptor-Mediated Transport of Insulin Across Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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