Key result
Endothelial cells rapidly release ~95% of bound insulin intact within 15 minutes.
Why the study?
The role of endothelial cells in the transport of biologically intact insulin and their potential function as an extra-pancreatic insulin storage site was unclear.
Population
Cultured endothelial cells derived from bovine pulmonary arteries
Comparison
Incubation with Mono A14-[125I]-iodoinsulin at physiologic conditions
Design
Preclinical experimental study
Authors
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Does not inform clinical insulin use; hypothesis-generating for endothelial insulin handling in vivo.
Cultured bovine pulmonary artery endothelial cells rapidly transport biologically intact insulin, suggesting a physiological role in transporting insulin out of the bloodstream or serving as an extra-pancreatic storage area.
DERNOVSEK et al. (1984) studied this question. Mono A14-[125I]-iodoinsulin was evaluated on Processing of cell-bound A14-[125I]-iodoinsulin. Approximately 95% of cell-bound Mono A14-[125I]-iodoinsulin dissociated from cultured bovine pulmonary artery endothelial cells in less than 15 minutes, passing through biologically intact.
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