What question did this study set out to answer?

To develop and evaluate a point-of-care electrochemical biosensor device capable of simultaneously measuring eight sepsis host response biomarkers directly in whole blood.

March 6, 2022Bioengineering & Translational MedicineOpen Access

Simultaneous detection of sepsis host response biomarkers in whole blood using electrochemical biosensor

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Authors

ATAmbalika Sanjeev Tanak ASAbha Sardesai SMSriram Muthukumar

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Overview

Diagnostic validation study demonstrates rapid multi-biomarker detection directly in whole blood from clinical samples, highlighting potential for point-of-care sepsis triage.

Key Points

To develop and evaluate a point-of-care electrochemical biosensor device capable of simultaneously measuring eight sepsis host response biomarkers directly in whole blood.
Engineered the DETecT Sepsis 2.0 sensor leveraging electrochemical impedance spectroscopy to detect IL-6, IL-8, IL-10, IP-10, TRAIL, d-dimer, CRP, and G-CSF using ~100 μl of undiluted whole blood.
Benchmarked the biosensor against standard laboratory Luminex assays using blood samples from 40 patients to evaluate clinical correlation and diagnostic accuracy.
Provided simultaneous quantification of all eight immune biomarkers in undiluted whole blood within 5 minutes.
Correlated strongly with reference standard Luminex measurements across clinical samples (r > 0.87).
Achieved diagnostic accuracy exceeding 80% with an area under the receiver operating characteristic curve (AUC) > 0.8.

Cite This Study

Tanak et al. (2022) studied this question.

synapsesocial.com/papers/69d7f28e11d83f35e5ae36c0 https://doi.org/10.1002/btm2.10310

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March 6, 2022Bioengineering & Translational MedicineOpen Access

Simultaneous detection of sepsis host response biomarkers in whole blood using electrochemical biosensor

View Full Paper

Ask AI

Bookmark

Authors

ATAmbalika Sanjeev Tanak ASAbha Sardesai SMSriram Muthukumar

Discussion

Member takes

Overview

Diagnostic validation study demonstrates rapid multi-biomarker detection directly in whole blood from clinical samples, highlighting potential for point-of-care sepsis triage.

Key Points

To develop and evaluate a point-of-care electrochemical biosensor device capable of simultaneously measuring eight sepsis host response biomarkers directly in whole blood.
Engineered the DETecT Sepsis 2.0 sensor leveraging electrochemical impedance spectroscopy to detect IL-6, IL-8, IL-10, IP-10, TRAIL, d-dimer, CRP, and G-CSF using ~100 μl of undiluted whole blood.
Benchmarked the biosensor against standard laboratory Luminex assays using blood samples from 40 patients to evaluate clinical correlation and diagnostic accuracy.
Provided simultaneous quantification of all eight immune biomarkers in undiluted whole blood within 5 minutes.
Correlated strongly with reference standard Luminex measurements across clinical samples (r > 0.87).
Achieved diagnostic accuracy exceeding 80% with an area under the receiver operating characteristic curve (AUC) > 0.8.

Cite This Study

Tanak et al. (2022) studied this question.

synapsesocial.com/papers/69d7f28e11d83f35e5ae36c0 https://doi.org/10.1002/btm2.10310

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