What question did this study set out to answer?

To determine whether myocardial cell hypertrophy occurs in neonatal rat ventricular cell cultures and to evaluate the regulatory effects of serum concentration and catecholamines on cell growth.

December 1, 1982Circulation Research

Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines.

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Population

Cultures from day-old rat ventricle, including serum-supplemented, bromodeoxyuridine-treated cultures and…

Comparison

Varying serum concentrations and chronic… vs Serum-free medium or baseline conditions.

Design

Preclinical

Follow-up

11 days

Authors

PSPaul SimpsonUniversity of North Carolina at Chapel Hill AMAiden McGrathUniversity of Massachusetts Amherst SSShoshana SavionTel Aviv University

Discussion

Member takes

Implication

Provides neonatal rat model for serum- and catecholamine-driven hypertrophy; leaves open translation to human disease.

Key Points

To determine whether myocardial cell hypertrophy occurs in neonatal rat ventricular cell cultures and to evaluate the regulatory effects of serum concentration and catecholamines on cell growth.
Cultured ventricular cells from day-old rats using either serum-supplemented medium with bromodeoxyuridine or serum-free medium containing transferrin and insulin, each achieving 75% to 80% myocyte predominance.
Quantified cell size across 11 days by measuring suspended cell volume via microscopic diameter, attached cell surface area via planimetry, and total cell protein via the Lowry method.
Tested the effects of serum titration (0% to 10%) and chronic exposure to 1 µM isoproterenol or norepinephrine with or without propranolol in myocytes and pure nonmyocardial cell controls.
In 5% serum cultures, myocyte size increased 2- to 3-fold over 11 days (final volume ~3,000 µm³/cell, surface area ~5,000 µm²/cell, and protein ~1,500 pg/cell), with overall size increasing ~4-fold from 0% to 5% or 10% serum.
Serum-free cultures exhibited no baseline hypertrophy but demonstrated dose-related hypertrophy upon chronic exposure to 1 µM norepinephrine or isoproterenol, an effect that was not blocked by equimolar propranolol.
Pure nonmyocardial cell controls exhibited time-dependent size increases that were unaffected by variations in serum concentration or the addition of norepinephrine.

Structured PICO

Population

Cultures from day-old rat ventricle, including serum-supplemented, bromodeoxyuridine-treated cultures and serum-free cultures with transferrin and insulin (about 75-80% myocardial cells).

Intervention

Varying serum concentrations (0%, 5%, 10%) and chronic exposure to catecholamines (isoproterenol or norepinephrine 1 microM).

Comparator

Serum-free medium or baseline conditions.

Outcome

Myocardial cell hypertrophy quantified by volume, surface area, and total cell protein concentration.surrogate

Myocardial cell hypertrophy can be induced in neonatal rat heart cultures by serum and catecholamines, providing an in vitro model to study nonhemodynamic factors of hypertrophy.

Cite This Study

Simpson et al. (1982) studied this question.

synapsesocial.com/papers/69de72fcfd84e72eb255855b https://doi.org/10.1161/01.res.51.6.787

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Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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