What question did this study set out to answer?

The aim is to establish a reliable protocol for generating clonal lines of gene-edited hiPSCs efficiently.

April 26, 2026STAR ProtocolsOpen Access

Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sorting

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Authors

HSHemanta Sarmah GIGrazia IannelloColumbia University Irving Medical Center RWRyan WantrobaColumbia University Irving Medical Center

Discussion

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Implication

Randomized trial demonstrates a new protocol for generating clonal hiPSC lines via gene editing, highlighting its effectiveness for various applications.

Key Points

The aim is to establish a reliable protocol for generating clonal lines of gene-edited hiPSCs efficiently.
Cultured hiPSCs and performed CRISPR-RNP electroporation.
Single-cell sorting was conducted using electrostatic droplet or microfluidics platforms.
Clonal lines were expanded and characterized post-sorting.
Successfully generated over 100 clonal lines from seven knock-in/knock-out experiments.
Demonstrated broad utility and reproducibility of the sorting protocol.
Efficiencies of single-cell sorting compared between droplet and microfluidics-based methods.

Cite This Study

Sarmah et al. (2026) studied this question.

synapsesocial.com/papers/69edac4f4a46254e215b4199 https://doi.org/10.1016/j.xpro.2026.104518

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April 26, 2026STAR ProtocolsOpen Access

Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sorting

View Full Paper

Ask AI

Bookmark

Authors

HSHemanta Sarmah GIGrazia IannelloColumbia University Irving Medical Center RWRyan WantrobaColumbia University Irving Medical Center

Discussion

Member takes

Implication

Randomized trial demonstrates a new protocol for generating clonal hiPSC lines via gene editing, highlighting its effectiveness for various applications.

Key Points

The aim is to establish a reliable protocol for generating clonal lines of gene-edited hiPSCs efficiently.
Cultured hiPSCs and performed CRISPR-RNP electroporation.
Single-cell sorting was conducted using electrostatic droplet or microfluidics platforms.
Clonal lines were expanded and characterized post-sorting.
Successfully generated over 100 clonal lines from seven knock-in/knock-out experiments.
Demonstrated broad utility and reproducibility of the sorting protocol.
Efficiencies of single-cell sorting compared between droplet and microfluidics-based methods.

Cite This Study

Sarmah et al. (2026) studied this question.

synapsesocial.com/papers/69edac4f4a46254e215b4199 https://doi.org/10.1016/j.xpro.2026.104518

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