Genome assembly and metagenome analysis identifies diverse microbial communities in a demosponge.
<ns3:p> We present a genome assembly from an individual <ns3:italic>Theonella swinhoei</ns3:italic> (demosponge; Porifera; Demospongiae; Tetractinellida; Theonellidae). The genome sequence has a total length of 144.15 megabases. Most of the assembly (98.21%) is scaffolded into 21 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 18.01 kilobases. Gene annotation of this assembly by Ensembl identified 18 129 protein-coding genes. The metagenome of the specimen was further assembled, and members of 19 bacterial and archaeal phyla were identified, among them Chloroflexota, Pseudomonadota, Acidobacteriota, and Actinobacteriota, as well as <ns3:italic>Ca.</ns3:italic> Tectomicrobia and <ns3:italic>Ca.</ns3:italic> Poribacteria. The 145 binned genomes included 69 high-quality MAGs, of which 47 were fully circularised. </ns3:p>
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