What does this research mean for the field?

Insulin is a potent negative regulator of IGFBP-2 mRNA expression in rat hepatocytes, whereas growth hormone has no effect on its levels. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to determine how insulin and growth hormone (GH) affect IGFBP-2 mRNA expression in rat hepatocytes.

September 1, 1990Molecular EndocrinologyOpen Access

Insulin Regulates the Expression of the Insulin-Like Growth Factor Binding Protein 2 mRNA in Rat Hepatocytes

View Full Paper

Ask AI

Bookmark

Why the study?

Does insulin or GH regulate IGFBP-2 mRNA expression in primary rat hepatocytes?

Population

Primary hepatocytes from adult rats

Comparison

Insulin or Growth Hormone (GH) vs Cells cultured in the absence of hormones

Design

Preclinical

Authors

MBMarianne Böni‐SchnetzlerUniversity Hospital of Basel CSChristoph SchmidUniversity Hospital of Zurich JMJ. Laws MaryUniversity Hospital of Basel

Discussion

Member takes

Implication

Should not yet inform insulin or GH therapy; hypothesis-generating for hepatic IGF regulation in humans.

Key Points

This research aims to determine how insulin and growth hormone (GH) affect IGFBP-2 mRNA expression in rat hepatocytes.
Primary hepatocytes from adult rats were cultured with and without hormones.
mRNA levels of IGFBP-2 and IGF l were measured to assess hormonal effects.
Concentration-response experiments evaluated the impact of insulin on IGFBP-2 mRNA levels.
IGFBP-2 mRNA was abundant in the absence of hormones but significantly reduced with insulin treatment.
Half-maximal down-regulation of IGFBP-2 mRNA occurred at insulin concentrations of 1 to 2 x 10(-10) M.
GH had no effect on IGFBP-2 mRNA levels, though it increased IGF l mRNA levels.

Structured PICO

Does insulin or GH regulate IGFBP-2 mRNA expression in primary rat hepatocytes?

Population

Primary hepatocytes from adult rats

Intervention

Insulin or Growth Hormone (GH)

Comparator

Cells cultured in the absence of hormones

Outcome

IGFBP-2 mRNA expression levelssurrogate

Insulin acts as a potent negative regulator of hepatic IGFBP-2 mRNA levels, suggesting a regulatory link between insulin and IGF-1.

Cite This Study

Böni‐Schnetzler et al. (1990) studied this question.

synapsesocial.com/papers/6a06cee0964d5135c0d3d089 https://doi.org/10.1210/mend-4-9-1320

View Full Paper

Ask AI

Bookmark

Insulin Regulates the Expression of the Insulin-Like Growth Factor Binding Protein 2 mRNA in Rat Hepatocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Insulin Regulates the Expression of the Insulin-Like Growth Factor Binding Protein 2 mRNA in Rat Hepatocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider