June 1, 1991Endocrinology

Multihormonal Regulation of Insulin-Like Growth Factor-Binding Protein-1 in Rat H4IIE Hepatoma Cells: The Dominant Role of Insulin*

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Structured PICO

Population

Rat H4IIE hepatoma cells stabilized in serum-free medium

Intervention

Insulin (100 nM), 8-(4-chlorophenylthio)cAMP (8-CPT-cAMP) (75 μM), dexamethasone (1 μM), rat GH, testosterone, progesterone, or 17β-estradiol

Comparator

Control (untreated cells in serum-free medium)

Outcome

IGFBP-1 production (measured by [125I]IGF-I binding assay, Western ligand and immunoblot analyses) and IGFBP-1 mRNA abundance (Northern and slot blot analysis)surrogate

Insulin plays a dominant role in downregulating IGFBP-1 production and mRNA abundance in rat hepatoma cells, counteracting the stimulatory effects of cAMP and dexamethasone.

Authors

TUTerry G. UntermanUniversity of Illinois Urbana-Champaign DOD. OehlerNorthwestern University LMLiam J. MurphyCreighton University

Discussion

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Implication

Insulin downregulation of IGFBP-1 in rat hepatoma cells is hypothesis-generating; leaves open translation to human IGF-I regulation and metabolic disease.

Cite This Study

Unterman et al. (1991) studied this question.

synapsesocial.com/papers/6a700df6a7fbea1e4407ee28 https://doi.org/10.1210/endo-128-6-2693

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Implication

Insulin downregulation of IGFBP-1 in rat hepatoma cells is hypothesis-generating; leaves open translation to human IGF-I regulation and metabolic disease.

Multihormonal Regulation of Insulin-Like Growth Factor-Binding Protein-1 in Rat H4IIE Hepatoma Cells: The Dominant Role of Insulin*

Structured PICO

Discussion

Implication

Cite This Study

Multihormonal Regulation of Insulin-Like Growth Factor-Binding Protein-1 in Rat H4IIE Hepatoma Cells: The Dominant Role of Insulin*

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider