What is the clinical evidence from this study?

Study design: Other. Population: Patients undergoing cardiac surgery. Intervention: Insulin vs. Basal state. Primary outcome: Stimulation of L-type calcium current (ICa,L).

What question did this study set out to answer?

This research aims to evaluate the effect of insulin on the L-type calcium current in human atrial myocytes.

November 1, 1999Cardiovascular ResearchOpen Access

Stimulation of L-type Ca2+ current in human atrial myocytes by insulin

View Full Paper

Ask AI

Bookmark

Key result

Insulin stimulated the L-type calcium current in isolated human atrial myocytes in a dose-dependent manner, with a maximum stimulation of 140 +/- 12% over basal at 10 µM.

Why the study?

Does insulin stimulate the L-type calcium current in isolated human atrial myocytes?

Population

Isolated human atrial myocytes obtained from patients undergoing cardiac surgery

Comparison

Insulin at concentrations of 100 nM, 1 microM… vs Basal L-type calcium current (ICa,L)

Design

Preclinical

Authors

SMSmaranda MaierClinical Emergency Hospital Bucharest

Discussion

Member takes

Implication

Does not yet inform insulin use in patients; hypothesis-generating for atrial electrophysiology in hyperinsulinemic states.

Key Points

This research aims to evaluate the effect of insulin on the L-type calcium current in human atrial myocytes.
Isolated human atrial myocytes were obtained from cardiac surgery patients.
The patch-clamp technique was used to measure the stimulation of L-type calcium current by insulin.
Concentration ranges of insulin (100 nM to 10 microM) were administered to assess dose-dependent effects.
Insulin resulted in a maximum stimulation of L-type calcium current by 140 +/- 12% at 10 microM (n=11).
Maximum conductance of L-type calcium current increased from 4.0 +/- 0.3 nS to 8.3 +/- 1.0 nS with 10 microM insulin (n=6).
The effect of insulin was reversed by acetylcholine and inhibited by a selective insulin receptor inhibitor.

Structured PICO

Does insulin stimulate the L-type calcium current in isolated human atrial myocytes?

Population

Isolated human atrial myocytes obtained from patients undergoing cardiac surgery

Intervention

Insulin at concentrations of 100 nM, 1 microM, and 10 microM

Comparator

Basal L-type calcium current (ICa,L)

Outcome

Stimulation of L-type calcium current (ICa,L)surrogate

Insulin stimulates the L-type calcium current in isolated human atrial myocytes in a dose-dependent and reversible manner, likely via the insulin receptor tyrosine kinase.

Cite This Study

Smaranda Maier (1999) studied Patients undergoing cardiac surgery. Insulin vs. Basal state was evaluated on Stimulation of L-type calcium current (ICa,L). Insulin stimulated the L-type calcium current in isolated human atrial myocytes in a dose-dependent manner, with a maximum stimulation of 140 +/- 12% over basal at 10 µM.

synapsesocial.com/papers/6a08098d113ba5b476ddec30 https://doi.org/10.1016/s0008-6363(99)00229-1

View Full Paper

Ask AI

Bookmark

Stimulation of L-type Ca2+ current in human atrial myocytes by insulin

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Stimulation of L-type Ca2+ current in human atrial myocytes by insulin

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider