Alterations in Myocardial Creatinine Kinase (CK) and Lactate Dehydrogenase (LDH) Isoenzyme-Distribution in a Model of Left Ventricular Dysfunction
Key result
Epinephrine-induced cardiomyopathy in rabbits was associated with decreased total LV lactate dehydrogenase activity (0.43 vs 0.52 IU/mg protein; P<0.05) and a shift towards anaerobic isoenzymes.
Why the study?
Does repetitive epinephrine infusion alter myocardial CK and LDH isoenzyme distribution in a rabbit model of LV dysfunction?
Population
16 rabbits (8 in epinephrine-induced cardiomyopathy model, 8 untreated controls)
Comparison
Four repetitive epinephrine infusions in 12-day… vs Untreated controls
Design
Preclinical
Authors
Discussion
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Implication
May indicate metabolic remodeling in catecholamine cardiomyopathy; hypothesis-generating for human HF studies.
Structured PICO
Does repetitive epinephrine infusion alter myocardial CK and LDH isoenzyme distribution in a rabbit model of LV dysfunction?
P
Population
16 rabbits (8 in epinephrine-induced cardiomyopathy model, 8 untreated controls)
I
Intervention
Four repetitive epinephrine infusions (300 mg/kg/60 min, i.v.) in 12-day intervals
C
Comparator
Untreated controls
O
Outcome
Myocardial creatinine kinase (CK) and lactate dehydrogenase (LDH) activity and isoenzyme distributionsurrogate
Main Result
Absolute Event Rate: 0.43% vs 0.52%
p-value: p=<0.05
In a rabbit model of catecholamine-induced cardiomyopathy, LV dysfunction is associated with impaired LDH activity, a shift towards anaerobic isoenzymes, and increased foetal B-CK isoenzymes, indicating changes in cardiac energy metabolism.
Cite This Study
Muders et al. (2001) studied Epinephrine-induced cardiomyopathy (n=16). Epinephrine infusion vs. Untreated controls was evaluated on Total LV lactate dehydrogenase activity (IU/mg protein) (p=<0.05). Epinephrine-induced cardiomyopathy in rabbits was associated with decreased total LV lactate dehydrogenase activity (0.43 vs 0.52 IU/mg protein; P<0.05) and a shift towards anaerobic isoenzymes.