What question did this study set out to answer?

To assess the redistribution of creatine kinase isoenzymes in rat ventricular myocardium undergoing hypertrophy induced by chronic pressure overload.

January 1, 1985Cardiovascular Research

Redistribution of creatine kinase isoenzymes in chronically overloaded myocardium

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To assess the redistribution of creatine kinase isoenzymes in rat ventricular myocardium undergoing hypertrophy induced by chronic pressure overload.
Induced chronic pressure overload and ventricular hypertrophy in rats via experimental abdominal aortic stenosis, comparing them against sham-operated controls.
Separated and quantified myocardial creatine kinase (CK) isoenzymes (MM, MB, and BB) using a two-step analytical procedure combining anion exchange chromatography and electrophoresis.
Overloaded ventricles showed a reduction in CK-MM activity from 407 ± 10 to 370 ± 0.13 μmol·min⁻¹·g⁻¹ wet weight (p < 0.05), alongside increases in CK-MB (from 56 ± 4 to 77 ± 6, p < 0.02) and CK-BB (from 6.5 ± 0.7 to 10.1 ± 1.2, p < 0.02).
Rats with severe hypertrophy (ventricular/body weight ratio > 3 mg/g) showed greater shifts to 318 ± 15 for MM, 83 ± 15 for MB, and 15.1 ± 2.5 for BB (p < 0.01).
Ventricular/body weight ratio positively correlated with total CK-MB levels (r = 0.32, p < 0.05) and with the percentage of B monomer subunits (r = 0.51, p < 0.01).

Structured PICO

Population

Rats with experimental abdominal aortic stenosis causing chronic myocardial overload

Intervention

Experimental abdominal aortic stenosis

Comparator

Sham-operated rats

Outcome

Creatine kinase (CK) isoenzyme profile (MM, MB, BB isoforms) in cardiac tissuesurrogate

Chronic myocardial overload in rats causes a shift in creatine kinase isoenzymes towards fetal forms (MB and BB), suggesting an adaptive mechanism in hypertrophied myocytes.

Discussion

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Implication

May represent adaptive remodeling in rat hypertrophy; leaves open relevance to human HF energetics.

Cite This Study

Younes et al. (1985) studied this question.

synapsesocial.com/papers/6a12fc968f1bac20a09ea7b5 https://doi.org/10.1093/cvr/19.1.15

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Redistribution of creatine kinase isoenzymes in chronically overloaded myocardium

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Redistribution of creatine kinase isoenzymes in chronically overloaded myocardium

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider