What question did this study set out to answer?

To evaluate total DNA content changes and define the relative contributions of nuclear polyploidy and mitotic cell division during acute and chronic cardiac hypertrophy.

October 1, 1969Circulation ResearchOpen Access

Biochemical Correlates of Cardiac Hypertrophy: III. CHANGES IN DNA CONTENT; THE RELATIVE CONTRIBUTIONS OF POLYPLOIDY AND MITOTIC ACTIVITY

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Why the study?

Does aortic constriction alter DNA content, polyploidy, and mitotic activity in the left ventricle of rats?

Population

Rats (3-month-old, 12- to 13-week-old, and 17- to 18-week-old)

Comparison

Aortic constriction vs Sham-operated litter mates or normal rats

Design

Preclinical

Follow-up

Up to 4 months

Authors

DGDavid GroveCerner (United States)KNK. G. NairWilliams & Associates RZRadovan ZakUniversity of Chicago

Discussion

Member takes

Implication

Hypothesis-generating in rat hypertrophy models; leaves open human relevance and clinical translation.

Key Points

To evaluate total DNA content changes and define the relative contributions of nuclear polyploidy and mitotic cell division during acute and chronic cardiac hypertrophy.
Induced cardiac hypertrophy in 12- to 18-week-old rats using surgical aortic constriction, comparing them to sham-operated littermates.
Measured left ventricular DNA concentration, nuclear polyploid frequency, and tissue mitotic indices at short-term (3 to 21 days) and chronic (2 to 4 months) post-operative time points.
Total ventricular DNA increased proportionally with acute heart enlargement (DNA concentration was 1.38 ± 0.03 µg/mg wet weight in both banded and sham rats), but DNA accumulated significantly less during chronic hypertrophy.
Nuclear polyploidy increased from 1.8% to 4.2% in 12- to 13-week-old rats at 1 to 3 weeks post-constriction, and from 1.6% to 3.9% in 17- to 18-week-old rats 10 days post-constriction.
Mitotic indices in hypertrophied hearts increased tenfold compared to controls during the first 3 weeks, with mitotic figures localized almost entirely in non-muscle cells.

Structured PICO

Does aortic constriction alter DNA content, polyploidy, and mitotic activity in the left ventricle of rats?

Population

Rats (3-month-old, 12- to 13-week-old, and 17- to 18-week-old)

Intervention

Aortic constriction

Comparator

Sham-operated litter mates or normal rats

Outcome

DNA concentration and content, percentage of polyploid nuclei, and mitotic indices in the left ventriclesurrogate

Cardiac hypertrophy induced by aortic constriction in rats is associated with proportional increases in DNA content, increased polyploidy, and significantly higher mitotic activity primarily outside muscle cells.

Cite This Study

Grove et al. (1969) studied this question.

synapsesocial.com/papers/6a0906d562c780efd627fbd4 https://doi.org/10.1161/01.res.25.4.463

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Biochemical Correlates of Cardiac Hypertrophy: III. CHANGES IN DNA CONTENT; THE RELATIVE CONTRIBUTIONS OF POLYPLOIDY AND MITOTIC ACTIVITY

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Biochemical Correlates of Cardiac Hypertrophy: III. CHANGES IN DNA CONTENT; THE RELATIVE CONTRIBUTIONS OF POLYPLOIDY AND MITOTIC ACTIVITY

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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