Key result
Transient expression of poliovirus 2Apro in COS-1 cells resulted in a 4-fold reduction in DNA replication and a 22-fold reduction in RNA polymerase II transcription compared to control plasmids.
Population
COS-1 monkey kidney cells
Comparison
Transient expression of poliovirus proteinase… vs Identical plasmid containing a translation…
Design
Preclinical
Authors
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2Apro suppresses host transcription and replication beyond p220; challenges single-pathway models and leaves open in vivo relevance.
Poliovirus proteinase 2Apro expression inhibits host protein synthesis primarily through a reduction in RNA polymerase II transcription and DNA replication, rather than just p220 cleavage.
Davies et al. (1991) studied Poliovirus infection mechanism. Transient expression of poliovirus proteinase 2Apro vs. Identical plasmid containing a translation termination codon within the 2Apro coding region was evaluated on DNA replication, RNA polymerase II transcription, and translation. Transient expression of poliovirus 2Apro in COS-1 cells resulted in a 4-fold reduction in DNA replication and a 22-fold reduction in RNA polymerase II transcription compared to control plasmids.
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