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May 17, 2026Journal of Molecular and Cellular Cardiology Plus0 citationsOpen Access

Acute sildenafil administration decreases the susceptibility to induced atrial fibrillation in sheep

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GMGeorge MaddersInsermNDNathan DenhamUniversity Health NetworkDHD.C. HutchingsUniversity of Manchester

Key Result

Intravenous sildenafil significantly reduced vulnerability to induced atrial fibrillation in sheep by decreasing both AF incidence and duration.

Key Points

  • This study investigates whether sildenafil can reduce the susceptibility to atrial fibrillation (AF) during electrophysiological testing in sheep.
  • In vivo electrophysiological studies were conducted in 12 anaesthetized sheep.
  • Sheep received 10 mg intravenous sildenafil before measurements of AF susceptibility.
  • AF incidence and duration, along with atrial electrophysiological parameters, were analyzed.
  • Sildenafil significantly reduced AF incidence and duration.
  • Increased atrial effective refractory period and atrial wavelength were observed.
  • Alternans magnitude decreased, supporting reduced AF vulnerability.

Structured PICO

Does 10 mg intravenous sildenafil reduce vulnerability to induced atrial fibrillation in anaesthetised sheep?

P
Population
12 anaesthetised sheep
I
Intervention
10 mg intravenous sildenafil
C
Comparator
Baseline (before sildenafil administration)
O
Outcome
Atrial fibrillation (AF) vulnerability (incidence and duration)surrogate

Acute intravenous sildenafil reduces vulnerability to induced atrial fibrillation in a sheep model by favorably altering atrial electrophysiology.

Limitations

  • The antiarrhythmic effects of acute sildenafil should be confirmed in man.

Abstract

Sildenafil has been shown to decrease ventricular arrhythmias. We investigated whether sildenafil could similarly decrease atrial fibrillation (AF) burden through direct effects on atrial electrophysiology. In vivo electrophysiological studies were performed in 12 anaesthetised sheep before and after 10 mg intravenous sildenafil. Sildenafil decreased AF vulnerability by decreasing both AF incidence and duration. The antiarrhythmic actions of sildenafil were associated with increases in atrial effective refractory period and atrial wavelength, together with a decrease in alternans magnitude which were expected to limit both AF duration and incidence respectively. The antiarrhythmic effects of acute sildenafil should be confirmed in man. • Intravenous sildenafil significantly reduced vulnerability to AF in sheep. • Sildenafil increased atrial effective refractory period and atrial wavelength. • Sildenafil also decreased alternans magnitude. • These effects are well-established as anti-arrhythmic. • The observed reduction of AF by sildenafil warrants further investigation in man.

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Cite This Study

Madders et al. (2026) studied Atrial fibrillation (n=12). Sildenafil vs. Baseline (before sildenafil) was evaluated on AF vulnerability (incidence and duration). Intravenous sildenafil significantly reduced vulnerability to induced atrial fibrillation in sheep by decreasing both AF incidence and duration.

synapsesocial.com/papers/6a095b3e7880e6d24efe0f16https://doi.org/10.1016/j.jmccpl.2026.100853
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