February 1, 1982Proceedings of the National Academy of Sciences

Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.

Q: What is the clinical evidence from this study?

Population: Hypertriglyceridemia/infection/shock (in vitro model). Intervention: Conditioned medium from endotoxin-treated exudate cells vs. Conditioned medium from untreated exudate cells or fresh medium. Primary outcome: Lipoprotein lipase activity.

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Key result

Conditioned medium from endotoxin-treated mouse peritoneal exudate cells suppressed lipoprotein lipase activity in differentiating 3T3-L1 mouse preadipocytes by greater than 90%.

Population

3T3-L1 mouse preadipocytes and mouse peritoneal exudate cells

Comparison

Conditioned medium from cultures of mouse… vs Endotoxin alone, conditioned medium from exudate…

Design

Preclinical

Authors

MKMasanobu KawakamiJichi Medical University PPP H PekalaBoston University MLM. Daniel LaneRutgers, The State University of New Jersey

Discussion

Member takes

Implication

May inform mechanisms of infection-related dyslipidemia in models; leaves open mediator identity and human relevance.

Key Points

The aim is to identify a mediator released from exudate cells that suppresses lipoprotein lipase activity in differentiating 3T3-L1 preadipocytes.
Conditioned medium from endotoxin-treated peritoneal exudate cells was used on 3T3-L1 cell cultures.
The impact on lipoprotein lipase activity was assessed after 30 minutes of exposure to the mediator-containing medium.
Various concentrations of insulin were tested for their ability to reverse the suppression.
The mediator resulted in greater than 90% suppression of lipoprotein lipase activity.
The suppression was not reversed by high concentrations of insulin, demonstrating the mediator's potency.
The mediator is heat labile with a molecular weight of at least 12,000 and does not inhibit enzyme activity directly.

Structured PICO

Population

3T3-L1 mouse preadipocytes and mouse peritoneal exudate cells

Intervention

Conditioned medium from cultures of mouse peritoneal exudate cells incubated with endotoxin

Comparator

Endotoxin alone, conditioned medium from exudate cells not exposed to endotoxin, or lysates of exudate cells

Outcome

Lipoprotein lipase (triacylglycero-protein acylhydrolase) activitysurrogate

Endotoxin promotes the release of a mediator from exudate cells that suppresses lipoprotein lipase activity in preadipocytes, providing a potential mechanism for lipid metabolism alterations during infection.

Cite This Study

Kawakami et al. (1982) studied Hypertriglyceridemia/infection/shock (in vitro model). Conditioned medium from endotoxin-treated exudate cells vs. Conditioned medium from untreated exudate cells or fresh medium was evaluated on Lipoprotein lipase activity. Conditioned medium from endotoxin-treated mouse peritoneal exudate cells suppressed lipoprotein lipase activity in differentiating 3T3-L1 mouse preadipocytes by greater than 90%.

synapsesocial.com/papers/6a0a31da36c3abab50464aa2 https://doi.org/10.1073/pnas.79.3.912

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Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider