What is the clinical evidence from this study?

Study design: Other. Population: Muscle differentiation. Intervention: Mlp60A and Mlp84B characterization.

What question did this study set out to answer?

To identify and characterize Drosophila homologs of the cysteine-rich LIM protein family and evaluate their expression and function during muscle development.

September 1, 1996The Journal of Cell BiologyOpen Access

Two muscle-specific LIM proteins in Drosophila.

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Key result

Drosophila muscle LIM proteins Mlp60A and Mlp84B are developmentally regulated and associate with the actin cytoskeleton, suggesting a role in myogenesis late in the differentiation pathway.

Population

Drosophila embryos and rat fibroblast cells

Design

Preclinical

Authors

BSBeth StronachAllegheny Health Network SSSarah E SiegristUniversity of Virginia MBMary C. BeckerleUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

May inform vertebrate myogenesis studies; leaves open translational relevance to human muscle disease.

Key Points

To identify and characterize Drosophila homologs of the cysteine-rich LIM protein family and evaluate their expression and function during muscle development.
Cloned and sequenced Drosophila cDNAs encoding muscle LIM proteins Mlp60A and Mlp84B.
Analyzed embryonic tissue distribution and developmental timing of Mlp expression using in situ localization and Northern blotting across life stages.
Expressed Mlp60A and Mlp84B in cultured rat fibroblast cells to examine subcellular interactions with the actin cytoskeleton.
Mlp60A encodes a single LIM domain linked to a glycine-rich region distributed throughout muscle fibers, whereas Mlp84B encodes five tandem LIM-glycine modules enriched at muscle attachment sites.
Northern blot analysis revealed a biphasic expression pattern restricted to somatic, visceral, and pharyngeal musculature, peaking during late embryogenesis and metamorphosis.
Both Mlp60A and Mlp84B associate directly with the actin cytoskeleton when expressed heterologously in rat fibroblasts.

Structured PICO

Population

Drosophila embryos and rat fibroblast cells

Intervention

Identification and characterization of muscle LIM proteins Mlp60A and Mlp84B

Outcome

Spatial and temporal expression patterns and actin cytoskeleton association of Mlp60A and Mlp84B

The temporal expression and spatial distribution of Mlp60A and Mlp84B in Drosophila suggest a role in myogenesis late in the differentiation pathway.

Cite This Study

Stronach et al. (1996) studied Muscle differentiation. Mlp60A and Mlp84B characterization was evaluated. Drosophila muscle LIM proteins Mlp60A and Mlp84B are developmentally regulated and associate with the actin cytoskeleton, suggesting a role in myogenesis late in the differentiation pathway.

synapsesocial.com/papers/6a0cc4d004164ea96334c2ed https://doi.org/10.1083/jcb.134.5.1179

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Two muscle-specific LIM proteins in Drosophila.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Two muscle-specific LIM proteins in Drosophila.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider