During in vitro differentiation of C2C12 mouse skeletal muscle cells, alpha-cardiac actin mRNA accumulated to levels six times higher than alpha-skeletal actin within 24 hours.
We examined the expression of alpha-skeletal, alpha-cardiac, and beta- and gamma-cytoskeletal actin genes in a mouse skeletal muscle cell line (C2C12) during differentiation in vitro. Using isotype-specific cDNA probes, we showed that the alpha-skeletal actin mRNA pool reached only 15% of the level reached in adult skeletal muscle and required several days to attain this peak, which was then stably maintained. However, these cells accumulated a pool of alpha-cardiac actin six times higher than the alpha-skeletal actin mRNA peak within 24 h of the initiation of differentiation. After cells had been cultured for an additional 3 days, this pool declined to 10% of its peak level. In contrast, over 95% of the actin mRNA in adult skeletal muscle coded for alpha-actin. This suggests that C2C12 cells express a pattern of sarcomeric actin genes typical of either muscle development or regeneration and distinct from that seen in mature, adult tissue. Concurrently in the course of differentiation the beta- and gamma-cytoskeletal actin mRNA pools decreased to less than 10% of their levels in proliferating cells. The decreases in beta- and gamma-cytoskeletal actin mRNAs are apparently not coordinately regulated.
Bains et al. (Wed,) conducted a other in Skeletal muscle cell differentiation. In vitro differentiation vs. Proliferating cells / adult skeletal muscle was evaluated on Expression of actin genes (alpha-skeletal, alpha-cardiac, beta- and gamma-cytoskeletal). During in vitro differentiation of C2C12 mouse skeletal muscle cells, alpha-cardiac actin mRNA accumulated to levels six times higher than alpha-skeletal actin within 24 hours.
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