Angiotensin II stimulated cell proliferation and decreased renin content in 3T3 mouse fibroblasts, whereas the antagonist saralasin inhibited cell growth and increased cellular renin concentration.
Angiotensin II stimulates cell proliferation and decreases intracellular renin in mouse fibroblasts, suggesting a role in cell growth regulation and a negative feedback mechanism.
Components of the renin-angiotensin system were studied in established cell culture lines of 3T3 and SV3T3 mouse fibroblasts. The renin content in 3T3 cells was significantly higher than in virus-transformed SV3T3 cells. With time after infection, renin decreased in Simian virus 40 transformed cells, while it increased steadily in mock-infected 3T3 cells. In contrast to renin, angiotensinase activity was higher in SV3T3 cells. Angiotensin II stimulated cell proliferation in 3T3 mouse fibroblasts and decreased their renin content in a dose-related manner. In contrast, saralasin, an angiotensin receptor antagonist, inhibited cell growth in 3T3 and SV3T3 cells and caused an increase of cellular renin concentration. The angiotensin fragments angiotensin (2-8) heptapeptide and angiotensin (4-8) pentapeptide had no effect on cell growth. A significant negative correlation was found between cell proliferation and renin levels in 3T3 and SV3T3 cells irrespective of the treatment. Our results indicate (1) that angiotensin II may be involved in cell growth regulation, (2) that a negative feedback exist between angiotensin II added and intracellular renin content, and (3) that virus infection causes a decrease in intracellular renin synthesis, while non-specific angiotensinase activity is increased under this condition.
Schelling et al. (Thu,) conducted a other in Cell culture (mouse fibroblasts). Angiotensin II and saralasin vs. Untreated / mock-infected cells was evaluated on Cell proliferation and renin content. Angiotensin II stimulated cell proliferation and decreased renin content in 3T3 mouse fibroblasts, whereas the antagonist saralasin inhibited cell growth and increased cellular renin concentration.