Effects of Angiotensin II on the Growth of Vascular Smooth Muscle Cells

Kyung Man Kim; In Seop Kim; Su Je Park; Wang Soo Lee; Hak Jin Kim; Sang‐Wook Kim; Tae Ho Kim; Chee Jeong Kim; Mi Kyung Kim; Wang Seong Ryu; Un Ho Ryoo

doi:10.4070/kcj.1999.29.2.209

January 1, 1999Sunhwan'gi

Effects of Angiotensin II on the Growth of Vascular Smooth Muscle Cells

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Why the study?

Does angiotensin II induce cellular hypertrophy or mitogenic activity in cultured rat aortic vascular smooth muscle cells?

Key Points

To examine the direct effects of angiotensin II on the growth, mitogenesis, and protein synthesis of cultured vascular smooth muscle cells.
Isolated rat aortic smooth muscle cells (passages 4–8) using combined enzymatic digestion with collagenase and elastase.
Treated cultured cells daily for 3 days with angiotensin II alone or combined with equimolar concentrations of saralasin.
Quantified protein and DNA synthesis by measuring [14C]phenylalanine and [3H]thymidine incorporation via liquid scintillation spectrometry.
Angiotensin II increased [14C]phenylalanine incorporation by approximately 20% to 30%, reflecting stimulated protein synthesis.
Saralasin completely blocked the angiotensin II-mediated increase in [14C]phenylalanine incorporation.
Angiotensin II stimulation produced no significant increase in [3H]thymidine incorporation, showing an absence of mitogenic activity.

Structured PICO

Does angiotensin II induce cellular hypertrophy or mitogenic activity in cultured rat aortic vascular smooth muscle cells?

P

Population

Cultured rat aortic smooth muscle cells (between 4th and 8th passages) obtained by combined collagenase and elastase methods

I

Intervention

Angiotensin II alone or angiotensin II with equimolar concentrations of saralasin, treated daily for 3 days

O

Outcome

Cellular hypertrophy and mitogenic activity measured by incorporated radioactivity of (3H)thymidine and (14C)phenylalaninesurrogate

Angiotensin II induces cellular hypertrophy but lacks detectable mitogenic activity in cultured rat aortic vascular smooth muscle cells.

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Implication

Ang II induces VSMC hypertrophy without mitogenesis in vitro; hypothesis-generating for vascular remodeling mechanisms.

Abstract

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Cite This Study

Kim et al. (1999) studied this question.

synapsesocial.com/papers/6a8d1a75b7e5f37e3ddacdf0 https://doi.org/10.4070/kcj.1999.29.2.209

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Effects of Angiotensin II on the Growth of Vascular Smooth Muscle Cells

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Effects of Angiotensin II on the Growth of Vascular Smooth Muscle Cells

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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