Key result
Labeling actin with NBD-Cl at Lysine 372 produced a 2.2- to 2.3-fold enhancement of fluorescence upon polymerization without affecting the polymerization rate or cofactor activity.
Population
N-ethylmaleimide actin
Design
Preclinical
Authors
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NBD-Cl actin labeling at Lys372 enables non-perturbing polymerization monitoring in vitro; leaves open validation for cardiovascular cell studies.
NBD-Cl labeling of actin at Lysine 372 provides a sensitive fluorescent probe for monitoring actin polymerization without altering its functional properties.
Detmers et al. (1981) studied Actin polymerization. 7-chloro-4-nitrobenzeno-2-oxa-1,3-diazole chloride (NBD-Cl) was evaluated on Fluorescence enhancement upon polymerization. Labeling actin with NBD-Cl at Lysine 372 produced a 2.2- to 2.3-fold enhancement of fluorescence upon polymerization without affecting the polymerization rate or cofactor activity.
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