Key result
Transient expression of rat Kv2.1 K+ channels in mammalian COS-1 cells yielded a 108 kDa phosphorylated protein exhibiting large, voltage-dependent outward currents.
Population
Mammalian COS-1 cells transfected with rat Kv2.1 K+ channel polypeptide
Comparison
Transient expression of rat Kv2.1 K+ channel… vs Xenopus oocytes microinjected with Kv2.1 cRNA
Design
Preclinical
Authors
Loading...
COS-1 cells enable Kv2.1 functional studies; leaves open translation to cardiovascular ion channel research.
Transfected mammalian COS-1 cells serve as an effective system for the rapid biochemical and electrophysiological analysis of functional Kv2.1 K+ channels.
Shi et al. (1994) studied this question. Transfection of mammalian COS-1 cells with rat Kv2.1 K+ channel polypeptide vs. Xenopus oocytes microinjected with Kv2.1 cRNA was evaluated on Properties of expressed Kv2.1 K+ channels. Transient expression of rat Kv2.1 K+ channels in mammalian COS-1 cells yielded a 108 kDa phosphorylated protein exhibiting large, voltage-dependent outward currents.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: