Induction of platelet-derived growth factor A-chain and c-myc gene expressions by angiotensin II in cultured rat vascular smooth muscle cells.
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Population
Cultured rat aortic smooth muscle cells made quiescent by 48-h exposure to a defined serum-free medium.
Comparison
Angiotensin II added to culture media vs Vehicle or Angiotensin II with saralasin or…
Design
Preclinical
Follow-up
up to 25 hours
Authors
Discussion
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Implication
Does not yet alter hypertension management; extends experimental evidence for Ang II-driven vascular hypertrophy mechanisms.
Key Points
- This study investigates how angiotensin II affects gene expressions related to cell growth in vascular smooth muscle cells.
- Used rat aortic smooth muscle cells made quiescent with serum-free medium for 48 hours.
- Treated cells with varying concentrations of angiotensin II (10(-6) to 10(-11) M) and measured gene expression over time.
- Examined effects of protein synthesis inhibitor cycloheximide on mRNA stability.
- Angiotensin II induced c-myc mRNA expression within 30 minutes, lasting 6 hours.
- PDGF A-chain mRNA expression increased by 15-20-fold, peaking at 9 hours and remaining elevated for 11 hours.
- Both effects were dose-dependent and inhibited by saralasin.
Structured PICO
P
Population
Cultured rat aortic smooth muscle (RASM) cells (passages 8-18) made quiescent by 48-h exposure to a defined serum-free medium.
I
Intervention
Angiotensin II (10^-6 to 10^-11 M) added to culture media
C
Comparator
Vehicle (control) or Angiotensin II with saralasin or cycloheximide
O
Outcome
Expression of c-myc and PDGF A-chain mRNA and PDGF concentration in culture mediumsurrogate
Angiotensin II sequentially activates c-myc and PDGF A-chain gene expressions in vascular smooth muscle cells, suggesting a molecular mechanism for angiotensin-induced vascular hypertrophy.
Cite This Study
Naftilan et al. (1989) studied this question.
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