Key result
Encephalomyocarditis virus RNA was detectable by PCR in murine hearts up to 90 days post-infection, persisting into the chronic stage of myocarditis when infectious virus could no longer be isolated.
Why the study?
Can viral RNA be detected by PCR in the chronic stage of EMCV-induced myocarditis in a murine model?
Population
Four-week-old DBA/2 mice and FL (human amnion) cells
Design
Preclinical
Follow-up
90 days
Authors
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Suggests PCR utility for probing viral persistence in chronic myocarditis models; leaves open relevance to human dilated cardiomyopathy.
Can viral RNA be detected by PCR in the chronic stage of EMCV-induced myocarditis in a murine model?
PCR can detect persistent viral RNA in the chronic stage of viral myocarditis even after infectious virus is no longer isolatable and inflammation has subsided, suggesting its utility in investigating viral etiologies of dilated cardiomyopathy.
Kyu et al. (1992) studied Dilated cardiomyopathy / viral myocarditis. Encephalomyocarditis virus (EMCV) inoculation was evaluated on Detection of viral RNA by PCR at various time points. Encephalomyocarditis virus RNA was detectable by PCR in murine hearts up to 90 days post-infection, persisting into the chronic stage of myocarditis when infectious virus could no longer be isolated.
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