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June 1, 1984Clinical Chemistry

High-density-lipoprotein cholesterol in heparin-MnCl2 supernates determined with the Dow enzymic method after precipitation of Mn2+ with HCO3-.

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Authors

PBPaul S. BachorikUniversity of MiamiRWRobert WalkerBoston UniversityDVDonna G. VirgilJohns Hopkins University

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Implication

Randomized trial finds improved HDL cholesterol measurement accuracy in heparin-Mn2+ supernates after NaHCO3 treatment, suggesting better diagnostic reliability.

Key Points

  • This study aims to evaluate the accuracy of enzymic HDL cholesterol measurements in heparin-Mn2+ supernates before and after treatment with NaHCO3.
  • Measured HDL cholesterol enzymically in heparin-Mn2+ supernates from 96 fresh plasma samples and 72 frozen sera.
  • Compared results with the automated Liebermann-Burchard method post-NaHCO3 treatment.
  • Analyzed bias in measurements independent of cholesterol, triglyceride, and HDL-cholesterol concentrations.
  • Enzymic HDL-cholesterol values for untreated supernates were 10.4% higher than comparison method values, decreasing to +2.3% with treatment.
  • For frozen sera, the enzymic values were 6.0% and 0.5% higher for untreated and treated supernates respectively.
  • Measurements agreed within 21 mg/L with CDC-established values for pooled serum controls.

Cite This Study

Bachorik et al. (1984) studied this question.

synapsesocial.com/papers/6a1074fad478ddac0ffcf82ahttps://doi.org/10.1093/clinchem/30.6.839
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Enzymatic determinations of cholesterol in high-density-lipoprotein fractions prepared by a precipitation technique.1976 · 174 citations
  2. 2A SIMPLIFIED METHOD FOR THE ESTIMATION OF TOTAL CHOLESTEROL IN SERUM AND DEMONSTRATION OF ITS SPECIFICITY1952 · 3,094 citations
  3. 3Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000.1981 · 211 citations
  4. 4Plasma high-density lipoprotein cholesterol concentrations determined after removal of other lipoproteins by heparin/manganese precipitation or by ultracentrifugation.1976 · 70 citations
  5. 5A comprehensive evaluation of the heparin–manganese precipitation procedure for estimating high density lipoprotein cholesterol1978 · 1,260 citations