What does this research mean for the field?

The coding regions of actin genes are highly conserved across species and tissues, whereas the 3' non-coding regions have diverged considerably during evolution, allowing probes from these 3' regions to specifically identify various actin mRNAs and genes. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To sequence and characterize the coding and 3′ untranslated regions of rat skeletal muscle actin mRNA and determine their evolutionary conservation across tissues and animal species.

January 1, 1981Nucleic Acids ResearchOpen Access

Skeletal muscle actin mRNA. Characterization of the 3′ untranslated region

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Population

Plasmids containing cDNA inserts complementary to rat skeletal muscle actin mRNA, and RNA extracted from…

Design

Preclinical

Authors

MSMoshe ShaniAgricultural Research Organization UNUri NudelWeizmann Institute of Science DZD. Zevin‐SonkinLos Alamos National Laboratory

Discussion

Member takes

Implication

3' non-coding probes enable specific actin isoform detection; extends molecular tools for cardiovascular research but leaves clinical translation open.

Key Points

To sequence and characterize the coding and 3′ untranslated regions of rat skeletal muscle actin mRNA and determine their evolutionary conservation across tissues and animal species.
Cloned and sequenced rat skeletal muscle actin cDNA plasmids p749 (codons 171–360), p150 (codons 357–374 plus 120 bp of 3′ UTR), and p106 (terminal codon and full 3′ UTR).
Performed RNA cross-hybridization assays across multiple rat tissues (skeletal, cardiac, smooth muscle, and brain) as well as dog, rabbit, and chick tissues.
Evaluated sequence conservation and divergence using hybrid thermal stability measurements and S1 nuclease digestion assays.
Plasmid p749, containing the actin coding region, hybridized broadly across all examined rat tissues and cross-hybridized strongly with dog and chick RNA.
Plasmid p106, spanning the 3′ untranslated region, hybridized specifically to striated muscle mRNA in rats, rabbits, and dogs, but failed to hybridize to rat non-muscle/smooth muscle RNA or chick RNA.
Thermal stability and S1 digestion profiles demonstrated substantial evolutionary divergence between the 3′ untranslated ends of rat and dog skeletal muscle actin mRNAs.

Structured PICO

Population

Plasmids (p749, p106, p150) containing cDNA inserts complementary to rat skeletal muscle actin mRNA, and RNA extracted from various tissues (rat skeletal, cardiac, and smooth muscle, brain; dog, chick, and rabbit tissues)

Intervention

Nucleotide sequence analysis and RNA hybridization

Outcome

Sequence relationships and hybridization specificity of actin mRNA regionssurrogate

Probes constructed from the 3' non-coding regions of actin mRNAs can be used to specifically identify various actin mRNA and actin genes due to their evolutionary divergence.

Cite This Study

Shani et al. (1981) studied this question.

synapsesocial.com/papers/6a11ffc20514fa642cccdbec https://doi.org/10.1093/nar/9.3.579

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Implication

3' non-coding probes enable specific actin isoform detection; extends molecular tools for cardiovascular research but leaves clinical translation open.

Skeletal muscle actin mRNA. Characterization of the 3′ untranslated region

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Skeletal muscle actin mRNA. Characterization of the 3′ untranslated region

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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