What question did this study set out to answer?

Investigate whether platelet membrane glycoprotein Ib serves as the primary receptor for von Willebrand factor using a specific inhibitory monoclonal antibody.

January 1, 1983Blood

Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor

View Full Paper

Ask AI

Bookmark

Investigate whether platelet membrane glycoprotein Ib serves as the primary receptor for von Willebrand factor using a specific inhibitory monoclonal antibody.
Produced a murine monoclonal antibody using hybridoma technology and isolated purified intact antibody and F(ab')2 fragments.
Evaluated platelet agglutination, electrophoretic mobility, prothrombin time, and binding parameters using radiolabeled fragments on normal platelets and platelets from 2 patients with Bernard-Soulier syndrome.
Identified the target receptor site using immunoprecipitation and affinity chromatography.
Antibody and F(ab')2 fragments completely blocked platelet agglutination induced by ristocetin and bovine von Willebrand factor and eliminated von Willebrand factor antigen binding.
Radiolabeled F(ab')2 fragments bound approximately 2.5 × 10^4 sites on normal platelets with high affinity (KD ~1.5 × 10^-8 M), with zero binding observed on Bernard-Soulier syndrome platelets.
The antibody localized to the externally oriented glycocalicin portion of the GPIb alpha chain, which copurified with an unidentified ~20,000 molecular weight molecule.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro study demonstrates complete inhibition of von Willebrand factor binding to human platelets by a monoclonal antibody, indicating glycoprotein Ib acts as the essential receptor.

Cite This Study

Coller et al. (1983) studied this question.

synapsesocial.com/papers/6a12656ef7bd4f5c7da63d8c https://doi.org/10.1182/blood.v61.1.99.99

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates complete inhibition of von Willebrand factor binding to human platelets by a monoclonal antibody, indicating glycoprotein Ib acts as the essential receptor.

Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor

Key Points

Discussion

Implication

Cite This Study

Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider