Effects of Long-Term Intervention with Losartan, Aspirin and Atorvastatin on Vascular Remodeling in Juvenile Spontaneously Hypertensive Rats

Figures

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  Figure 1. The histological morphological changes in thoracic aorta in each group (22 weeks rats). (A) H&E stain in cross section of thoracic aortas. (scale bar = 300 μm); (B) magnified images of (A) (scale bar = 50 μm); (C) wall thickness (WT), wall-to-lumen ratio (WLR), inner diameter (ID) and number of VSMCs (VSMC) in tunica media, respectively. The data are presented as the mean ± SD. n = 6, ## p < 0.01 vs. WKY; * p < 0.05, ** p < 0.01 vs. SHR-M.

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  Figure 2. The histological morphological changes in carotid aorta in each group (22 weeks rats). (A) H&E stain in cross section of carotid aortas. (scale bar = 200 μm); (B) magnified images of (A) (scale bar = 100 μm); (C) wall thickness (WT), wall-to-lumen ratio (WLR), inner diameter (ID), and number of VSMCs (VSMC) in tunica media, respectively. The data are presented as the mean ± SD. n = 6, ## p < 0.01 vs. WKY; * p < 0.05, ** p < 0.01 vs. SHR-M.

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  Figure 3. Thoracic aortas (A,B) or carotid arteries (C,D) elastin by H&E stain under fluorescence microscope showed intense red autofluorescence (22 weeks rats). (B,D) are enlarged views of (A,C), respectively (A: scale bar = 300 μm; B: scale bar = 50 μm; C: scale bar = 200 μm; D: scale bar = 50 μm); (E) counts of link across elastin fiber layers in thoracic aortas; (F) counts of link across elastin fiber layers in carotid arteries. The data are presented as the mean ± SD. n = 6, ## p < 0.01 vs. WKY; ** p < 0.01 vs. SHR-M.

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  Figure 4. The distribution of collagen fibers in cross sections of thoracic aortas (22 weeks rats). (A) Sirius Red stain of collagen fibers under optical microscope (scale bar = 300 μm); (B) magnified images of (A) (scale bar = 100 μm); (C) Sirius Red stain of collagen I and collagen III under polarized light microscopy, collagen I was bright red or strong orange–red, and collagen III was green (scale bar = 100 μm); (D) the percentage of collagen fibers in thoracic aortas. The data are presented as the mean ± SD. n = 6, # p < 0.05, vs. WKY.

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  Figure 5. The distribution of collagen in cross sections of carotid arteries (22 weeks rats). (A) Sirius Red stain of collagen under optical microscope (scale bar = 200 μm); (B) magnified images of (A) (scale bar = 100 μm); (C) Sirius Red stain of collagen I and collagen III under polarized light microscopy, collagen I was bright red or strong orange–red, and collagen III was green (scale bar = 100 μm); (D) the percentage of collagen fibers in carotid arteries. The data are presented as the mean ± SD. n = 6.

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  Figure 6. Effects of losartan, aspirin, and atorvastatin on the plasma contents of renin (A), Ang II (B), and ALD (C) in 22 weeks rats. The data are presented as the mean ± SD. n = 6, ## p < 0.01 vs. WKY; * p < 0.05, ** p < 0.01 vs. SHR-M.

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  Figure 7. Effects of losartan, aspirin, and atorvastatin on the plasma contents of ET-1 (A), NE (B), and IL-6 (C) in 22 weeks rats. The data are presented as the mean ± SD. n = 6, ## p < 0.01 vs. control (WKY); * p < 0.05, ** p < 0.01 vs. SHR-M group.