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January 1, 1983Nucleic Acids ResearchOpen Access

Molecular cloning and nucleotide sequence of a human renin cDNA fragment

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Population

Human kidney tissue, human chorionic tissue, and human DNA

Comparison

Molecular cloning and nucleotide sequencing of… vs Mouse submaxillary gland (SMG) renin cDNA

Design

Preclinical

Authors

FSFlorent SoubrierVascular MedicineJPJean‐Jacques PanthierCentre National de la Recherche ScientifiquePCPierre CorvolGeneral / Preventive / Lipids

Discussion

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Implication

Successful human renin cDNA cloning enables molecular studies; leaves open clinical translation for renin-angiotensin disorders.

Key Points

  • Clone and characterize human kidney renin messenger RNA and cDNA to investigate sequence conservation, tissue expression, and gene copy number.
  • Hybridized human kidney messenger RNA with a mouse submaxillary gland renin cDNA probe to isolate a 1.1-kilobase cDNA clone.
  • Compared human and mouse nucleotide sequences and performed Northern and Southern blot analyses to examine renin expression in chorionic tissue and determine gene copy number.
  • Identified human kidney renin mRNA at approximately 1.6 kb in length and isolated a 1.1 kb kidney renin cDNA clone.
  • Sequence analysis revealed conservation of catalytic residues and a potential glycosylation site between mouse and human renin.
  • Southern blot analysis demonstrated the presence of a single renin gene in human DNA, with chorionic renin mRNA showing a size similar to kidney renin mRNA.

Structured PICO

P
Population
Human kidney tissue, human chorionic tissue, and human DNA
I
Intervention
Molecular cloning and nucleotide sequencing of human renin cDNA
C
Comparator
Mouse submaxillary gland (SMG) renin cDNA
O
Outcome
Nucleotide sequence of human renin cDNA and comparison with mouse cDNAsurrogate

This basic science study successfully cloned and sequenced a human renin cDNA fragment, demonstrating structural conservation with mouse renin and confirming the presence of a single renin gene in the human genome.

Cite This Study

Soubrier et al. (1983) studied this question.

synapsesocial.com/papers/6a130f0ff7bd4f5c7da74679https://doi.org/10.1093/nar/11.20.7181
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A Radioimmunoassay for the Direct Measurement of Renin in Mice and Its Application to Submaxillary Gland and Kidney Studies1974 · 96 citations
  2. 2Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-γ 2a heavy chain1980 · 81 citations
  3. 3Human Chorionic Cells in Primary Culture: A Model for Renin Biosynthesis1982 · 99 citations
  4. 4Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA1980 · 2,689 citations
  5. 5Multiple forms of human renin. Purification and characterization.1979 · 141 citations