Key result
OTAC reduces LVFS by ~52% versus sham in mice.
Why the study?
Conventional suture-based transverse aortic constriction depends heavily on operator skill, necessitating an optimized and simplified method to induce cardiac pressure overload.
Does O-ring-induced transverse aortic constriction (OTAC) effectively induce cardiac hypertrophy and heart failure in mice?
Population
C57BL/6J mice
Comparison
OTAC with 0.50 mm vs 0.45 mm inner diameter o-rings
Design
Animal experimental study
Authors
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OTAC supports reproducible murine pressure-overload HF modeling; leaves open validation across strains before wider research adoption.
Does O-ring-induced transverse aortic constriction (OTAC) effectively induce cardiac hypertrophy and heart failure in mice?
Absolute Event Rate: 25.6% vs 53.2%
p-value: p=<0.05
OTAC is a simple, reproducible, and effective method for inducing cardiac pressure overload, hypertrophy, and heart failure with reduced ejection fraction in mice without requiring intubation.
Nakao et al. (2022) studied Cardiac hypertrophy and heart failure (n=84). O-ring-induced transverse aortic constriction (OTAC) vs. Sham operation was evaluated on Left ventricular fractional shortening (LVFS) at 8 weeks (p=<0.05). O-ring-induced transverse aortic constriction (OTAC) using a 0.45 mm o-ring significantly reduced left ventricular fractional shortening to 25.6% compared to 53.2% in sham-operated mice at 8 weeks.
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