What is the clinical evidence from this study?

Study design: Other. Intervention: Angiotensin II. Primary outcome: Activation of mitogen-activated protein kinase (MAPK).

What does this research mean for the field?

Angiotensin II stimulates multiple pathways to MAPK activation via PKC and ras/raf-1 kinase in bovine adrenal glomerulosa cells. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To identify the intracellular signaling pathways through which angiotensin II promotes mitogen-activated protein kinase activation and cellular growth in bovine adrenal glomerulosa cells.

April 1, 1998EndocrinologyOpen Access

Angiotensin II Activates Mitogen-Activated Protein Kinase Via Protein Kinase C and Ras/Raf-1 Kinase in Bovine Adrenal Glomerulosa Cells

View Full Paper

Ask AI

Bookmark

Key result

Angiotensin II stimulates multiple pathways to MAPK activation via PKC and ras/raf-1 kinase in bovine adrenal glomerulosa cells.

Population

Cultured bovine adrenal glomerulosa cells

Design

Preclinical

Authors

YTY. TianNational Institutes of Health

Discussion

Member takes

Implication

Extends Ang II mitogenic signaling mechanisms in adrenal cells; leaves open translation to human disease.

Key Points

To identify the intracellular signaling pathways through which angiotensin II promotes mitogen-activated protein kinase activation and cellular growth in bovine adrenal glomerulosa cells.
Stimulated cultured bovine adrenal glomerulosa cells with angiotensin II via the AT1 receptor across varying concentrations.
Assessed MAPK, Ras, and Raf-1 kinase activation while modifying intracellular and extracellular calcium levels and using pertussis toxin.
Investigated the necessity and sufficiency of protein kinase C (PKC) by stimulating cells with phorbol 12-myristate 13-acetate (PMA) and evaluating responses following PKC depletion.
Angiotensin II caused rapid, transient activation of MAPK with an ED50 of 10–50 pM through a predominantly pertussis toxin-insensitive pathway, with calcium serving a permissive role.
Angiotensin II induced rapid activation of Ras and Raf-1 kinase, whose activation kinetics closely matched MAPK activation.
PKC activation with PMA stimulated both MAPK and Raf-1 kinase, but PKC depletion selectively attenuated Ang II-induced MAPK activation without inhibiting Raf-1 kinase activation.

Structured PICO

Population

Cultured bovine adrenal glomerulosa cells

Intervention

Angiotensin II (Ang II)

Outcome

Activation of mitogen-activated protein kinase (MAPK)surrogate

Angiotensin II stimulates multiple pathways to MAPK activation via PKC and ras/raf-1 kinase in bovine adrenal glomerulosa cells.

Cite This Study

Y. Tian (1998) studied this question. Angiotensin II was evaluated on Activation of mitogen-activated protein kinase (MAPK). Angiotensin II stimulates multiple pathways to MAPK activation via PKC and ras/raf-1 kinase in bovine adrenal glomerulosa cells.

synapsesocial.com/papers/6a156d98d64fa333899fa840 https://doi.org/10.1210/en.139.4.1801

View Full Paper

Ask AI

Bookmark

Angiotensin II Activates Mitogen-Activated Protein Kinase Via Protein Kinase C and Ras/Raf-1 Kinase in Bovine Adrenal Glomerulosa Cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Angiotensin II Activates Mitogen-Activated Protein Kinase Via Protein Kinase C and Ras/Raf-1 Kinase in Bovine Adrenal Glomerulosa Cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider