Key result
An indo-1 ratiometric video technique allowed the imaging of ratio changes of intracellular calcium with a high temporal (1 ms) and spatial (0.6 mm) resolution in the isolated beating rat heart.
Population
isolated Tyrode-perfused beating rat heart loaded with indo-1
Design
Preclinical
Authors
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Enables high-resolution calcium studies in isolated hearts; leaves open translation to in vivo or clinical settings.
The study demonstrates a novel optical ratio imaging setup capable of high spatiotemporal resolution for beat-to-beat intracellular calcium imaging in isolated beating rat hearts.
Eerbeek et al. (2004) studied Intracellular calcium imaging in isolated rat heart. Optical ratio imaging setup and analysis method for indo-1 fluorescence was evaluated on Spatiotemporal resolution and signal-to-noise ratio of Ca(i)(2+) transients. An indo-1 ratiometric video technique allowed the imaging of ratio changes of intracellular calcium with a high temporal (1 ms) and spatial (0.6 mm) resolution in the isolated beating rat heart.
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