What is the clinical evidence from this study?

Study design: Other. Intervention: Simultaneous measurement system.

What question did this study set out to answer?

To develop a system for simultaneously measuring calcium concentration, cell contraction, and membrane potential in cardiac myocytes.

February 1, 1990AJP Heart and Circulatory Physiology

Simultaneous measurement of Ca2+, contraction, and potential in cardiac myocytes

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Key result

A described system enables simultaneous measurement of cytosolic Ca2+ concentration, cell length, and membrane potential or current in single cardiac myocytes without cross talk.

Population

Single cardiac myocytes loaded with the fluorescent Ca2+ indicator indo-1

Design

Preclinical

Authors

HSHarold A. SpurgeonElectrophysiology MSMichael D. SternElectrophysiology GBG. BaartzNational Institute on Aging

Discussion

Member takes

Implication

Supports advanced single-myocyte Ca2+ studies; leaves open integration with clinical electrophysiology.

Key Points

To develop a system for simultaneously measuring calcium concentration, cell contraction, and membrane potential in cardiac myocytes.
Simultaneous fluorescence recordings of Ca2+ using indo-1 with a flash illumination system.
Cell elongation measured via bright-field imaging during fluorescence collection.
Membrane potential or current recorded using patch-clamping techniques alongside indo-1 fluorescence.
The system allows concurrent measurement of [Ca2+]i with precise gating of time during light flashes.
Cell shortening and [Ca2+]i are recorded without cross talk due to separated light wavelengths.
Quantitative measurements of [Ca2+]i can be enhanced with free acid indo-1 by microinjection.

Structured PICO

Population

Single cardiac myocytes loaded with the fluorescent Ca2+ indicator indo-1

Intervention

Simultaneous measurement system using epi-illumination, dual-band fluorescence detection, bright-field imaging, and patch-clamping

Outcome

Simultaneous recording of cytosolic Ca2+ concentration, cell length, and membrane potential or current

Describes a novel methodology for the simultaneous measurement of calcium transients, contraction, and electrophysiology in isolated cardiac myocytes.

Limitations

Quantitation of [Ca2+]i is not precise with cells loaded with the permeant acetoxymethyl ester of indo-1 due to subcellular compartmentation.
Quantitation of [Ca2+]i is not precise with cells loaded with the permeant acetoxymethyl ester of indo-1 due to subcellular compartmentation

Cite This Study

Spurgeon et al. (1990) studied this question. Simultaneous measurement system was evaluated. A described system enables simultaneous measurement of cytosolic Ca2+ concentration, cell length, and membrane potential or current in single cardiac myocytes without cross talk.

synapsesocial.com/papers/6a6d3d8535aa2c282cdfd68a https://doi.org/10.1152/ajpheart.1990.258.2.h574

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Simultaneous measurement of Ca2+, contraction, and potential in cardiac myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Simultaneous measurement of Ca2+, contraction, and potential in cardiac myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider