June 1, 1992AJP Heart and Circulatory Physiology
Novel technique to load indo-1 free acid into single adult cardiac myocytes to assess cytosolic Ca2+
Key result
Novel indo-1 free acid loading yields peak cytosolic Ca2+ of 435 nM in rat ventricular cells.
- n=44
Population
Rat ventricular cells (n=44 for quiescent, n=43 for steady-state, n=42 for postrest)
Comparison
Loading indo-1 'free acid' selectively into the… vs Time-matched non-indo-loaded cells
Design
Preclinical
Authors
Discussion
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Implication
May refine Ca2+ imaging in rodent myocytes; leaves open translation to other models or clinical use.
Structured PICO
P
Population
In vitro study of 44 rat ventricular cells to assess cytosolic Ca2+ using a novel indo-1 loading technique.
I
Intervention
Loading indo-1 'free acid' selectively into the cytosol
C
Comparator
Time-matched non-indo-loaded cells (for autofluorescence subtraction)
O
Outcome
Cytosolic Ca2+ concentration ([Ca2+]i)surrogate
This novel method allows for the selective loading of indo-1 free acid into the cytosol of cardiac myocytes to accurately assess intracellular calcium concentrations.
Limitations
- Wide variation in the degree of uncertainty of individual intracellular Ca2+ concentration determinations among cells
Cite This Study
Sollott et al. (1992) studied this question. Indo-1 free acid loading vs. Time-matched non-indo-loaded cells was evaluated on Cytosolic [Ca2+]i. A novel method to load indo-1 free acid into rat ventricular cells yielded weighted mean cytosolic Ca2+ concentrations of 138 nM in quiescent cells and 435 nM at the transient peak.