Key result
In living embryonic cardiomyocytes transfected with a-actinin-GFP, premyofibrils containing Z-bodies formed at spreading edges and subsequently fused to form mature myofibrils with Z-bands.
Population
Cultured live embryonic cardiomyocytes
Design
Preclinical
Follow-up
several days in culture
Authors
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Extends fixed-cell models of myofibrillogenesis to live embryonic cardiomyocytes; leaves open relevance to mammalian cardiac development.
Live-cell imaging of embryonic cardiomyocytes supports the model that de novo fibrils near the cell membrane act as precursors (premyofibrils) to mature myofibrils.
Dabiri et al. (1997) studied Myofibrillogenesis. Transfection with a-actinin-GFP plasmid vs. Untransfected cells or GFP alone was evaluated on Visualization of myofibril assembly and Z-band formation. In living embryonic cardiomyocytes transfected with a-actinin-GFP, premyofibrils containing Z-bodies formed at spreading edges and subsequently fused to form mature myofibrils with Z-bands.
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