March 1, 1998AJP Heart and Circulatory Physiology
Measurement of heart rate and Q-T interval in the conscious mouse
Population
6 FVB mice (3 male, 3 female)
Design
Preclinical
Follow-up
24 hours
Authors
Discussion
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Member takes
Implication
Ketamine anesthesia confounds murine QT assessment; conscious telemetry extends transgenic ion channel models with lower variability.
Key Points
- To evaluate heart rate and Q-T intervals in conscious mice using telemetry, and relate them to cardiac function.
- Developed a telemetry system for electrocardiogram recording in conscious mice.
- Measured R-R and Q-T intervals from 1, 2, or 4 seconds of data every hour for 24 hours.
- Analyzed the relationship between Q-T and R-R intervals while examining effects of ketamine anesthesia.
- The Q-T interval correlated with the square root of the R-R interval (specific formula derived).
- Ketamine anesthesia significantly increased R-R and Q-T interval duration and variability.
- Low variability in Q-T across animals and time indicates the model's sensitivity for detecting changes in transgenic mice.
Structured PICO
P
Population
6 FVB mice (3 male, 3 female)
I
Intervention
Implantable telemetry system for ECG recording and analysis
O
Outcome
Measurement of R-R and Q-T intervalssurrogate
An implantable telemetry system in conscious mice provides a sensitive model with low variability for detecting changes in the Q-T interval, avoiding the confounding effects of anesthesia.
Cite This Study
Mitchell et al. (1998) studied this question.