What does this research mean for the field?

Chronic stimulation of predominantly fast skeletal muscle induces a complete switch in Ca-ATPase isozyme expression from type II (fast) to type I (slow) and significantly reduces calcium uptake activity. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine how chronic electrical stimulation of fast skeletal muscle alters the expression and functional activity of sarcoplasmic reticulum Ca-ATPase isozymes.

January 1, 1990FEBS LettersOpen Access

Ca‐ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

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Why the study?

Does chronic stimulation alter Ca-ATPase isozyme expression in fast skeletal muscle?

Population

Predominantly fast skeletal muscle

Comparison

Chronic stimulation vs Control muscles (unstimulated)

Design

Preclinical

Authors

FBF. Norman BriggsVirginia Commonwealth University Medical Center KLK.Francis LeeVirginia Commonwealth University Medical Center JFJoseph FeherVirginia Commonwealth University

Discussion

Member takes

Implication

Animal data on stimulation-induced Ca-ATPase remodeling warrant caution before clinical use; extends mechanistic understanding of muscle plasticity but leaves translation open.

Key Points

To determine how chronic electrical stimulation of fast skeletal muscle alters the expression and functional activity of sarcoplasmic reticulum Ca-ATPase isozymes.
Applied chronic electrical stimulation to predominantly fast skeletal muscles.
Identified type I (slow) and type II (fast) Ca-ATPase isozymes in isolated sarcoplasmic reticulum via Western blotting and in muscle fibers via immunohistochemistry using monoclonal antibodies IID8 and IIH11.
Measured Ca-ATPase and calcium-uptake activities in whole muscle homogenates and isolated sarcoplasmic reticulum fractions.
Immunohistochemical staining transitioned from 80% type II and 20% type I fibers in control muscles to 100% type I and 0% type II fibers following chronic stimulation.
Calcium uptake activity in stimulated muscle homogenates decreased to 22% of control muscle values.
Isolated sarcoplasmic reticulum from stimulated muscles exhibited Ca-ATPase and calcium-uptake activities that were 32% and 45% of control levels, respectively.

Structured PICO

Does chronic stimulation alter Ca-ATPase isozyme expression in fast skeletal muscle?

Population

Predominantly fast skeletal muscle

Intervention

Chronic stimulation

Comparator

Control muscles (unstimulated)

Outcome

Expression of type I (slow) and type II (fast) Ca-ATPase isozymessurrogate

Chronic stimulation of fast skeletal muscle induces a complete shift from fast (type II) to slow (type I) Ca-ATPase isozyme expression, accompanied by reduced calcium uptake activity.

Cite This Study

Briggs et al. (1990) studied this question.

synapsesocial.com/papers/6a198605ff42a97fac58396f https://doi.org/10.1016/0014-5793(90)80025-e

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Implication

Animal data on stimulation-induced Ca-ATPase remodeling warrant caution before clinical use; extends mechanistic understanding of muscle plasticity but leaves translation open.

Ca‐ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Ca‐ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider