What question did this study set out to answer?

To investigate the specificity of a monoclonal antibody to Ca2+-ATPase in canine cardiac and skeletal muscle fibers.

January 1, 1988Cell Motility and the CytoskeletonOpen Access

A monoclonal antibody to the Ca2+ ‐ATPase of cardiac sarcoplasmic reticulum cross‐reacts with slow type I but not with fast type II canine skeletal muscle fibers: An immunocytochemical and immunochemical study

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Monoclonal antibody (II D8) to the Ca2+-ATPase of canine cardiac sarcoplasmic reticulum vs. Monoclonal antibody (II H11) to skeletal type II Ca2+-ATPase. Primary outcome: Cross-reactivity and subcellular distribution of Ca2+-ATPase.

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Key result

A monoclonal antibody to the Ca2+-ATPase of canine cardiac sarcoplasmic reticulum cross-reacts with type I (slow) but not type II (fast) canine skeletal muscle fibers.

Population

Cryostat sections from three different adult canine skeletal muscles and cardiac tissue

Design

Preclinical

Authors

AJA O JorgensenUniversity of Copenhagen WAW ArnoldUniversity of Toronto DPD. R. PepperUniversity of Iowa

Discussion

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Implication

No clinical implications from this animal model; leaves open isoform-specific SR Ca2+-ATPase regulation for further study.

Key Points

To investigate the specificity of a monoclonal antibody to Ca2+-ATPase in canine cardiac and skeletal muscle fibers.
Immunofluorescence labeling of Ca2+-ATPase in cryostat sections from canine skeletal muscles
Characterization through immunoblotting to verify antibody binding
Comparison of labeling between type I (slow) and type II (fast) myofibers.
Type I myofibers exhibited strong immunofluorescence labeling for Ca2+-ATPase (p<0.001), whereas type II showed low, background labeling.
Immunoblotting revealed a single protein band of 115,000 Mr bound by monoclonal antibody II D8 in cardiac and type I myofiber extracts.
Epitopes on Ca2+-ATPase relevant for phosphorylated phospholamban interaction were confirmed to be shared between cardiac and type I muscle.

Structured PICO

Population

Cryostat sections from three different adult canine skeletal muscles (gracilis, extensor carpi radialis, and superficial digitalis flexor) and cardiac tissue

Exposure

Immunofluorescence labeling and immunoblotting with monoclonal antibodies (II D8 to cardiac Ca2+-ATPase, II H11 to skeletal type II Ca2+-ATPase)

Outcome

Localization and cross-reactivity of Ca2+-ATPase in different muscle fiber typessurrogate

The Ca2+-ATPase of cardiac and type I (slow) skeletal sarcoplasmic reticulum share a common epitope not found in type II (fast) skeletal muscle, possibly related to phospholamban interaction.

Cite This Study

Jorgensen et al. (1988) studied this question. Monoclonal antibody (II D8) to the Ca2+-ATPase of canine cardiac sarcoplasmic reticulum vs. Monoclonal antibody (II H11) to skeletal type II Ca2+-ATPase was evaluated on Cross-reactivity and subcellular distribution of Ca2+-ATPase. A monoclonal antibody to the Ca2+-ATPase of canine cardiac sarcoplasmic reticulum cross-reacts with type I (slow) but not type II (fast) canine skeletal muscle fibers.

synapsesocial.com/papers/6a6b380345e10bb5bcf2e20a https://doi.org/10.1002/cm.970090208

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A monoclonal antibody to the Ca2+ ‐ATPase of cardiac sarcoplasmic reticulum cross‐reacts with slow type I but not with fast type II canine skeletal muscle fibers: An immunocytochemical and immunochemical study

Discussion

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Implication

Key Points

Structured PICO

Cite This Study

A monoclonal antibody to the Ca2+ ‐ATPase of cardiac sarcoplasmic reticulum cross‐reacts with slow type I but not with fast type II canine skeletal muscle fibers: An immunocytochemical and immunochemical study

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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