March 1, 1994Circulation
Cardiac sarcoplasmic reticulum calcium ATPase, an autoimmune antigen in experimental cardiomyopathy.
Why the study?
Does immunization with cardiac SR-ATPase or its specific antibody induce experimental cardiomyopathy and myocarditis in mice?
Population
CAF1/J mice (n=11) and severe combined immunodeficiency (SCID) mice (n=15, including 3 controls)
Comparison
Immunization with purified canine cardiac… vs SCID mice receiving equivalent injections of…
Design
Preclinical
Follow-up
up to 12 weeks
Authors
Discussion
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Implication
Supports SR-ATPase as murine myocarditis autoantigen; hypothesis-generating for human autoimmunity, no practice change.
Structured PICO
Does immunization with cardiac SR-ATPase or its specific antibody induce experimental cardiomyopathy and myocarditis in mice?
P
Population
CAF1/J mice (n=11) and severe combined immunodeficiency (SCID) mice (n=15, including 3 controls)
I
Intervention
Immunization with purified canine cardiac SR-ATPase protein (CAF1/J mice) or intraperitoneal injection of 2 x 10^6 hybridoma cells producing 4C11-20.21 antibody (SCID mice)
C
Comparator
SCID mice receiving equivalent injections of hybridoma cells producing IgM anti-myosin light chain antibody
O
Outcome
Development of myocardial lesions, myocarditis, and cardiac myocyte degenerationsurrogate
Immunization with cardiac SR-ATPase or its specific antibody induces myocardial lesions and myocyte degeneration in mice, suggesting SR-ATPase is an autoimmunogen capable of inducing autoimmune myocarditis.
Cite This Study
Sharaf et al. (1994) studied this question.