Key result
Monoclonal antibodies inhibit sarcoplasmic reticulum Ca2+ uptake by ~95%, requiring the enzyme's oligomeric form.
Why the study?
Monoclonal antibodies to the Ca2+-ATPase of dog heart sarcoplasmic reticulum were produced to investigate their inhibitory effects on enzyme activity and structure.
Population
Purified sarcoplasmic reticulum vesicles from dog heart and 12 hybridoma clones
Comparison
Monoclonal antibodies vs no antibody control
Design
Preclinical experimental study
Authors
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Antibody inhibition of oligomeric SR Ca2+-ATPase in dogs is hypothesis-generating; leaves open any role in human heart failure therapy.
Monoclonal antibodies against dog heart SR Ca2+-ATPase inhibit its activity only when the enzyme is in an oligomeric form, affecting the whole active center.
Levitsky et al. (1987) studied this question. Monoclonal antibodies to Ca2+-ATPase was evaluated on Ca2+ uptake and ATPase activity. Monoclonal antibodies to dog heart sarcoplasmic reticulum markedly inhibited Ca2+ uptake by 95% and ATPase activity by 80%, requiring the enzyme to be in an oligomeric form.
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