What does this research mean for the field?

Physiological fluid shear stress rapidly downregulates endothelin-1 (ET-1) mRNA expression and peptide secretion in vascular endothelium, a response that depends on both the magnitude and dynamic character of the shear stress. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine how physiological levels and distinct dynamic patterns of fluid shear stress regulate endothelin-1 mRNA expression and peptide secretion in aortic endothelial cells.

August 1, 1992AJP Cell Physiology

Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium

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Why the study?

Does physiological fluid shear stress reduce endothelin-1 mRNA expression in bovine aortic endothelial cells?

Population

Bovine aortic endothelial cells grown in vitro

Comparison

Fluid shear stress of physiological magnitude vs No shear stress, cyclical stretch, or…

Design

Preclinical

Follow-up

1 hour

Authors

AMAli MalekTufts Medical Center SISeigo IzumoHeart Failure & Transplant

Discussion

Member takes

Implication

Hypothesis-generating for hemodynamic ET-1 control in endothelium; leaves open in vivo human relevance and clinical translation.

Key Points

To determine how physiological levels and distinct dynamic patterns of fluid shear stress regulate endothelin-1 mRNA expression and peptide secretion in aortic endothelial cells.
Cultured bovine aortic endothelial cells in vitro and exposed them to defined magnitudes of fluid shear stress, media viscosity modifications using dextran, and cyclical mechanical stretch.
Tested diverse hemodynamic flow profiles, including steady laminar shear (up to >15 dyn/cm2), pulsatile shear, turbulent shear, and low-frequency reversing shear stress.
Fluid shear stress caused a rapid (within 1 h), dose-dependent, fivefold decrease in ET-1 mRNA that saturated above 15 dyn/cm2 and reduced secreted ET-1 peptide levels.
Steady laminar, pulsatile, and turbulent shear stress of equal time-average magnitude (15 dyn/cm2) elicited identical decreases in ET-1 mRNA, whereas low-frequency reversing shear stress and cyclical stretch produced no change.

Structured PICO

Does physiological fluid shear stress reduce endothelin-1 mRNA expression in bovine aortic endothelial cells?

Population

Bovine aortic endothelial cells grown in vitro

Intervention

Fluid shear stress of physiological magnitude (steady laminar, pulsatile, and turbulent)

Comparator

No shear stress, cyclical stretch, or low-frequency reversing shear stress

Outcome

Level of endothelin-1 (ET-1) mRNAsurrogate

Physiological fluid shear stress downregulates endothelin-1 mRNA expression in vascular endothelium, demonstrating that hemodynamic forces directly modulate the expression of vasoactive peptides.

Cite This Study

Malek et al. (1992) studied this question.

synapsesocial.com/papers/6a1ab4bb9fa30811a0b902cc https://doi.org/10.1152/ajpcell.1992.263.2.c389

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Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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