Right Heart Failure in Mice Upon Pressure Overload Is Promoted by Mitochondrial Oxidative Stress

Figures

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  Figure 1. Right Ventricular Morphology and Function in 6J Mice Upon Pulmonary Artery Banding (A) Schematic illustration of experimental design. The pulmonary artery (PA) of mice was constricted to 450 μm (mild), 360 μm (moderate), or 300 μm (severe) using titanium clips. Right ventricular (RV) function and morphology were determined 2 weeks after pulmonary artery banding (PAB). (B) Representative 2-dimensional parasternal short-axis view of the constricted PA 3 days after PAB. Color Doppler visualizes blood flow, aortic valve (AV), pulmonic valve (PV), and proximal right ventricular outflow tract (pRVOT). (C) RV hypertrophy reflected by Fulton index (n = 10/8/9/10 mice) and diastolic right ventricular wall thickness (RVWT, d) (n = 13/8/9/10 mice) increased with increasing stenosis grade. (D) Representative M-mode images depicting assessment of RVWT, d and diastolic right ventricular inner diameter (RVID, d) of an untreated (Ctrl) and PAB-exposed mouse. (E) RVID, d and (F) right atrial (RA) area increased with stenosis grade of PAB (n = 13/8/9/10 mice). (G) Number of mice with paradoxical motion of interventricular septum was increased with increasing stenosis grade (n = 8/9/10 mice). (H)…

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  Figure 2. RV Function in 6J and 6N Mice Upon PAB (A) Representative genotyping of 6J and 6N mice by specific amplification of the nicotinamide nucleotide transhydrogenase (Nnt). 6J mice harbor an in-frame 5 exon deletion leading to a loss of exon 7-11.26 Primer pair 1 (PP1) is located within the coding sequence of exon 7-11, failing to be amplified in 6J mice. Primer pair 2 (PP2) is spanning exon 7-11, resulting in a truncated fragment in 6J mice compared with the wild type Nnt fragment amplified in 6N mice. (B) Schematic demonstration of NNT function. According to Nickel et al,18 the NNT switches from forward to reversed mode under cardiac pressure overload. (C) Schematic demonstration of experimental design. PAB was performed in 6J mice lacking the NNT (NntT/T) and 6N mice expressing functional active NNT (Nnt+/+). (D) RV hypertrophy as assessed by Fulton index (n = 11/4/10/9/13/8 mice) and diastolic RV wall thickness (n = 13/13/25/17/13/8 mice) was increased upon severe PAB after 2 and 4 weeks in 6J and 6N mice. Dilation of the RV (E) and the RA (F) was augmented in 6N compared with 6J mice upon 2 and 4 weeks of PAB (n = 13/13/25/17/13/8 mice). (G) RV function as assessed from TAPSE was…

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  Figure 3. Characteristics of Right Heart Failure Upon PAB (A) Macroscopic and (B and C) microscopic signs of venous congestion in livers were more pronounced in 6N compared with 6J mice upon 4 weeks of PAB (n = 8-14 mice). Representative images of livers upon PAB (A) after excision and (B) of liver sections stained with hematoxylin/eosin. Pale areas indicate damage caused by venous congestion. Scale bar = 50 μm. (D) Receiver-operating characteristic (ROC) curve for the area of hepatic venous congestion (C) of 6J and 6N mice receiving a cutoff value of 21.5%. (E) TAPSE of 6J and 6N mice upon 4 weeks of PAB showed a significant negative correlation and (F) RA area showed a significant positive correlation with the extent of hepatic venous congestion as assessed in hematoxylin/eosin-stained liver sections (n = 15 mice). (G) Survival rate, excluding animals that died at the day of surgery, was lower within 4 weeks after PAB in 6N compared with 6J mice (n = 14/13 mice). Statistical significance in C was calculated with 2-tailed unpaired Student’s t-test and in G with log-rank test. For E and F, Pearson correlation was tested. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Abbreviations as in Figure 1.

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  Figure 4. RV Remodeling in 6J and 6N Mice Upon Pulmonary Artery Banding (A) Representative images of picrosirius red stained RV sections (scale bar = 200 μm) and morphometric analysis of fibrotic area showing increased fibrosis in 6J and 6N mice upon PAB. (B) The extent of hepatic venous congestion did not correlate with RV fibrotic area in 6J and 6N mice upon 4 weeks of PAB (n = 16 mice). (C) Representative images of staining for cardiomyocyte cross-sectional area analysis using wheat germ agglutinin (WGA) and capillary density using isolectin of griffonia simplicifolia (GSI-B4). Scale bar = 50 μm. (D) Cross-sectional area of RV cardiomyocytes was not significantly different between 6J and 6N mice after 4 weeks of PAB. (E) Capillary density after 4 weeks of PAB in relation to the respective untreated animals of 6J and 6N mice shows slightly decreased density upon PAB in 6N compared with 6J mice. (F) The extent of hepatic venous congestion did not correlate with cardiomyocyte cross-sectional area in 6J and 6N mice upon 4 weeks of PAB (n = 16 mice). Statistical significance was calculated with 1-way analysis of variance followed by Bonferroni’s post hoc test for A, D and E. n = 4/4/8/8…

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  Figure 5. Temporal Development of RV Morphology, Function, and Failure in 6N Mice Upon PAB (A) Schematic demonstration of experimental design. PAB was performed in 6N mice expressing functional active NNT (Nnt+/+) and final organ harvest was performed at different time points. (B) RVID, d, (C) RA area, (D) TAPSE, and (E) RVWT, d were assessed after 1, 2, 4, and 6 weeks of PAB and were significantly increased after 1 week compared with baseline. (C) Area RA was further increased after 2, 4, and 6 weeks compared with 1 week, respectively (n = 15/20/34/22/11 mice). (F) Hepatic venous congestion as assessed from hematoxylin/eosin-stained sections (G) was significantly higher after 2, 4, and 6 weeks compared with 1 week of PAB (n = 6/9/8/11 mice). (H) The extent of RV fibrosis as assessed from picrosirius red-stained sections (I) was highest after 4 weeks of PAB (n = 6/9/8/11 mice). Statistical significance for B to E was calculated with a marginal linear mixed effect model and for F and H with 1-way analysis of variance followed by Bonferroni’s post hoc test. ∗∗P < 0.01; ∗∗∗P < 0.001 compared with respective control; §P < 0.05; §§P < 0.01; §§§P < 0.001 compared with the 1-week time point; &&&P…

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  Figure 6. Oxidative Stress-mediated Alterations in 6J and 6N Mice Upon Pulmonary Artery Banding Amount of hyperoxidized peroxiredoxin (Prx-SO2/3) in RV tissue normalized to protein expression of cyclooxygenase IV (COX IV) (A) was increased in 6N but not in 6J mice upon 4 weeks of PAB (n = 7/7/13/8 mice), and (B) the increase was significantly higher in 6N than in 6J mice as assessed by immunoblot (n = 13/8 mice). Oxidative DNA modifications as reflected by immunoreactivity for 8-hydroxydeoxyguanosin (8-OHdG) were (C and D) significantly increased in nuclei of cardiomyocytes upon 4 weeks of PAB compared with untreated in 6N mice, but not in 6J mice, and (E and F) were significantly more abundant in total nuclei of RV sections of 6N compared with 6J mice upon PAB (n = 4/4/7/8 mice). Arrowheads in C indicate cardiomyocyte nuclei, with 8-OHdG immunoreactivity in purple. Light blue color in E indicates immunoreactivity for 8-OHdG marked with ImageJ hue threshold analysis. Scale bar = 50 μm. (G) Mitochondrial (mt) copy number was slightly decreased upon PAB to the same extent in 6J and 6N mice (n = 7/4/9/8 mice). (H) Number of apoptotic nuclei calculated per 10 high-power fields (HPF) (40×…

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  Figure 7. Remodeling and Oxidative Damage in RV Tissue of Patients With Dilated Cardiomyopathy Before Heart Transplantation Patients with dilated cardiomyopathy were divided into 2 groups with normal systolic right ventricular function (nRVF) and severely impaired right ventricular function (iRVF), exhibiting significant differences in (A) TAPSE (n = 9/10), (B) fractional area change (FAC) (n = 11/10), (D) mean pulmonary arterial (PA) pressure (n = 11/10), and (E) pulmonary artery wedge pressure (PAWP) (n = 11/10). (C) Systolic LV ejection fraction was reduced without differences between the 2 groups (n = 8/9). (F) Pulmonary vascular resistance (PVR) was markedly increased in iRVF patients (n = 11/10). (G) RV-PA coupling reflected by the ratio of TAPSE to systolic pulmonary artery pressure (sPAP) was significantly impaired in iRVF patients (n = 9/9). (H) Percentage of fibrotic area as assessed from picrosirius red staining (scale bar = 100 μm) was not different in RV of nRVF and iRVF patients (n = 11/10). (I) Representative images of RV sections stained for cardiomyocyte cross-sectional area analysis using wheat germ agglutinin (WGA) and capillary density using isolectin of griffonia…