What does this research mean for the field?

S-nitrosoglutathione (GSNO) inhibits smooth muscle L-type Ca2+ channels through intracellular S-nitrosation of the α1 subunit's sulfhydryl groups, a mechanism that alters channel gating and conductance independently of nitric oxide release and guanylyl cyclase stimulation. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 1, 2001Journal of Biological ChemistryOpen Access

S-Nitrosation Controls Gating and Conductance of the α1 Subunit of Class C L-type Ca2+ Channels

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Population

Human embryonic kidney 293 expression system expressing smooth muscle L-type Ca2+ channels

Comparison

Extracellular administration of… vs Non-thiol nitric oxide donors…

Design

Preclinical

Authors

MPMichael PoteserMedical University of Vienna CRChristoph RomaninElectrophysiology WSWolfgang SchreibmayerCalifornia Institute of Technology

Discussion

Member takes

Implication

May indicate direct S-nitrosation pathway for Ca2+ channel regulation in smooth muscle; extends prior observations but leaves open human relevance.

Structured PICO

Population

Human embryonic kidney 293 expression system expressing smooth muscle L-type Ca2+ channels (class C, CaV1.2b) (either α1C-b alone or coexpressed with β2a and α2-δ)

Intervention

Extracellular administration of S-nitrosoglutathione (GSNO) 2 mM

Comparator

Non-thiol nitric oxide (NO) donors 2,2-diethyl-1-nitroso-oxhydrazin (2 mM) and 3-morpholinosydnonimine-hydrochloride (2 mM)

Outcome

Whole-cell Ba2+ currents and single-channel behavior (open probability and conductance)surrogate

GSNO modulates smooth muscle L-type Ca2+ channels via intracellular S-nitrosation, independent of NO release, highlighting a direct mechanism for Ca2+ channel regulation.

Cite This Study

Poteser et al. (2001) studied this question.

synapsesocial.com/papers/6a1beac64cc49ccc94a8f7e6 https://doi.org/10.1074/jbc.m008244200

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Implication

May indicate direct S-nitrosation pathway for Ca2+ channel regulation in smooth muscle; extends prior observations but leaves open human relevance.

S-Nitrosation Controls Gating and Conductance of the α1 Subunit of Class C L-type Ca2+ Channels

Discussion

Member takes

Implication

Structured PICO

Cite This Study

S-Nitrosation Controls Gating and Conductance of the α1 Subunit of Class C L-type Ca2+ Channels

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider