What does this research mean for the field?

Group II intron domain 5 is essential for cleavage at the 5' splice site and facilitates trans-splicing through a previously unpredicted interaction, whereas domain 4 is dispensable. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

June 1, 1988Molecular and Cellular BiologyOpen Access

Group II Intron Domain 5 Facilitates a trans-Splicing Reaction

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Population

Yeast mitochondrial DNA (aI5g) self-splicing group II intron in vitro model

Design

Preclinical

Authors

KJKevin A. JarrellModular Genetics (United States)RDRosemary C. DietrichCleveland Clinic PPPhilip S. PerlmanHoward Hughes Medical Institute

Discussion

Member takes

Implication

Supports minimal RNA constructs for splicing studies; leaves open validation in mammalian systems before any translational applications.

Structured PICO

Population

Yeast mitochondrial DNA (aI5g) self-splicing group II intron in vitro model

Intervention

Division within intron domain 4 and provision of domain 5 as a trans-acting RNA

Outcome

trans-splicing reaction and cleavage at the 5' splice site

Intron domain 5 is essential for cleavage at the 5' splice site in group II introns and can facilitate trans-splicing.

Cite This Study

Jarrell et al. (1988) studied this question.

synapsesocial.com/papers/6a1bef25ea84844e355f3154 https://doi.org/10.1128/mcb.8.6.2361-2366.1988

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Group II Intron Domain 5 Facilitates a trans-Splicing Reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Group II Intron Domain 5 Facilitates a trans-Splicing Reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider